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Arcturusxt lcm system

Manufactured by Thermo Fisher Scientific
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The ArcturusXT LCM System is a laser capture microdissection (LCM) instrument designed for the precise isolation of specific cells or tissue regions from heterogeneous samples. It enables researchers to collect pure, homogeneous cell populations for downstream molecular analysis.

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Lab products found in correlation

Picopure dna extraction kit, by Thermo Fisher Scientific (4 mentions) Qubit dsdna hs assay kit, by Thermo Fisher Scientific (4 mentions) Pen membrane slides, by Thermo Fisher Scientific (3 mentions) Capsure hs lcm cap, by Thermo Fisher Scientific (3 mentions) Picopure rna isolation kit, by Thermo Fisher Scientific (2 mentions) Capsure macro lcm cap, by Thermo Fisher Scientific (2 mentions) Qubit 2, by Thermo Fisher Scientific (2 mentions) Nebnext ultra 2 q5 master mix, by Thermo Fisher Scientific (2 mentions) Miseq nano platform, by Illumina (2 mentions) Agencourt ampure xp bead, by Beckman Coulter (2 mentions) Ampure xp bead, by Beckman Coulter (2 mentions) Minelute pcr purification kit, by Qiagen (2 mentions) Pen membrane glass slides, by Thermo Fisher Scientific (1 mentions) 2100 bioanalyzer, by Agilent Technologies (1 mentions) Histo clear 2, by National Diagnostics (1 mentions) Agilent rna 6000 pico kit, by Agilent Technologies (1 mentions) Histogene staining solution, by Thermo Fisher Scientific (1 mentions) N8010611, by Thermo Fisher Scientific (1 mentions) Rlt plus buffer, by Qiagen (1 mentions)

Close spelling variants of this product

ArcturusXT LCM ArcturusXT System ArcturusXT

16 protocols using arcturusxt lcm system

1

Laser Capture Microdissection of Tumor Cells

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Laser capture microdissection (LCM) of tumor cells and corresponding stroma was performed with the Applied Biosystems ArcturusXT LCM System, using Arcturus polyethylene naphthalate (PEN) Membrane Glass slides (Applied Biosystems, Life Technologies, UK) following staining with free RNase H&E. An average of 8,00,000–10,00,000 μm2 of tumor cells and stroma surface were separately captured and immediately frozen at −80°C prior to subsequent manipulation.
Diaz-Riascos Z.V., Ginesta M.M., Fabregat J., Serrano T., Busquets J., Buscail L., Cordelier P, & Capellá G. (2019). Expression and Role of MicroRNAs from the miR-200 Family in the Tumor Formation and Metastatic Propensity of Pancreatic Cancer. Molecular Therapy. Nucleic Acids, 17, 491-503.
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2

Laser Capture Microdissection of Plant Cells

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Paradermal sections of 7 µm thickness were prepared with a Reichert‐Jung microtome and mounted on PEN membrane glass slides (Applied Biosystems) with DEPC‐treated water. Steedman’s wax was removed by incubating slides in 100% acetone for 1 min, then Laser capture microdissection was performed on ArcturusXT LCM System (Applied Biosystems) following the user manual. Bundle sheath strands and mesophyll cells were harvested on CapSure Macro LCM Caps (Applied Biosystems), RNA was extracted using PicoPure RNA Isolation Kit with on‐column DNase treatment according to manufacturer's instructions. RNA quality was examined using Bioanalyzer 2100 (Agilent, Santa Clara) in combination with RNA pico chip.
Lee D., Hua L., Khoshravesh R., Giuliani R., Kumar I., Cousins A., Sage T.L., Hibberd J.M, & Brutnell T.P. (2021). Engineering chloroplast development in rice through cell‐specific control of endogenous genetic circuits. Plant Biotechnology Journal, 19(11), 2291-2303.
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3

Genomic Features of Lung Adenosquamous Carcinoma

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Thirty-three patients diagnosed with primary lung adenosquamous carcinoma (ASC) underwent surgical resection at Shanghai Chest Hospital between September 2017 and November 2019. Of these 33 patients, 14 had lymph node metastasis and three had multiple primary lesions of pure adenocarcinoma or SCC at diagnosis, which were surgically resected. Primary ASC samples were marked for adenocarcinoma and squamous components based on Hematoxylin and Eosin (H&E) staining and expression of IHC markers, including TTF1, Napsin A, p40, and CK5/6 and rigorously confirmed by two experienced pathologists. Then, laser-capture microdissection (LCM) was performed on all ASC sample sections using the ArcturusXT™ LCM system (Applied Biosystems). In addition, surgical samples from seven patients diagnosed with EGFR-positive primary LUSC were collected for sequencing to compare their genomic features with other EGFR-positive subtypes and EGFR wild-type LUSCs. This study has been reviewed and approved by the Shanghai Chest Hospital Research Ethics Committee (KS1851). All patients have signed written informed consent forms for genomic profiling.
Zhao R., Xu Y., Chen Y., Zhang J., Teng F., Liao S., Chen S., Wu Q., Xiang C., Pang J., Shang Z., Zhao J., Bao H., Bao H., Shao Y., Lu S, & Han Y. (2023). Clonal dynamics and Stereo-seq resolve origin and phenotypic plasticity of adenosquamous carcinoma. NPJ Precision Oncology, 7, 80.
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4

Laser Capture Microdissection of Pancreatic Islets

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In order to detect the mRNA expression level of target genes in the islets of the pancreas, we used a LCM technique to isolate the islets of the pancreas from mice in our study. The pancreatic tissues from Men1 KO and WT mice were harvested and embedded in OCT. The OCT-embedded tissue sections were stained by an Arcturus Histogene Frozen Section Staining Kit (AB Applied Biosystems, CA, USA) and the islet tissues dissected using an Arcturus XT LCM system.
Yuan Z., Gardiner J.C., Maggi E.C., Huang S., Adem A., Li G., Lee S., Slegowski D., Exarchakis A., Howe J.R., Lattime E.C., Zang X, & Libutti S.K. (2021). B7 immune-checkpoints as targets for the treatment of neuroendocrine tumors. Endocrine-related cancer, 28(2), 135-149.
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5

Laser Capture Microdissection of Immune Cells

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Immuno-stained cell populations; SIV p28+ virally infected cells and CD68+ macrophages in BM and CD163+ CNS perivascular macrophages were laser microdissected using an ArcturusXT™ LCM System and operating software (Applied Biosystems). A capsure HS polymer cap (Applied Biosystems) was placed on a tissue region containing immuno-stained cells and a minimum of 400 cells were captured. Infrared (IR) laser shots (18–20 µm diameter) were fired at 70 mV, capturing cells onto the polymer of the HS capsure cap. The time between immuno-staining and IR capture was kept to a maximum of 30 minutes to minimize RNA degradation.
Mallard J., Papazian E., Soulas C., Nolan D.J., Salemi M, & Williams K.C. (2017). A Method for Obtaining Simian Immunodeficiency Virus RNA Sequences from Laser Capture Microdissected and Immune Captured CD68+ and CD163+ Macrophages from Frozen Tissue Sections of Bone Marrow and Brain. Journal of immunological methods, 442, 59-63.
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6

Laser Capture Microdissection of Arabidopsis Seedlings

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Arabidopsis seedlings were fixed in Farmer’s fixative (3:1 ethanol:acetic acid) overnight at 4∘C. Subsequently, fixation, dehydration, and paraffin infiltration were performed using a microwave processor (H2850 EBS). Paraffin-embedded sections were cut to a thickness of 12 μm and mounted on a PEN membrane glass slide (Applied Biosystems LCM0522). To remove the paraffin, slides were immersed in Histo-Clear II (National Diagnostics HS-202; 2 × 5 min) and then air-dried at room temperature. Laser capture microdissection was performed using the ArcturusXT LCM system (Applied Biosystems). Selected areas were captured by an infrared laser onto Arcturus CapSure Macro LCM Cap (LCM0211 Applied Biosystems) and subsequently cut with a UV laser. Total RNA was extracted using the PicoPure RNA Isolation Kit (Applied Biosystems KIT0204) and quantified using the Agilent RNA 6000 Pico Kit (Agilent 5067-1513).
Matsunaga W., Ohama N., Tanabe N., Masuta Y., Masuda S., Mitani N., Yamaguchi-Shinozaki K., Ma J.F., Kato A, & Ito H. (2015). A small RNA mediated regulation of a stress-activated retrotransposon and the tissue specific transposition during the reproductive period in Arabidopsis. Frontiers in Plant Science, 6, 48.
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7

Laser Capture Microdissection of Tumor and Stromal Cells

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To investigate let-7 expression in tumor cells separately from stromal cells, 10 cases were selected, and paired tumor cells and tumor stromal tissues were obtained, using the Arcturus XT LCM system (Applied Biosystems, San Diego, CA). Sections were cut to 8 μm thickness, and placed on Arcturus polyethylene naphthalate membrane frame slides (catalog number, LCM0521) and allowed to dry. Based on histopathological review, tumor cells and adjacent stromal areas were separately micro-dissected and captured onto CapSure Macro LCM Caps (catalog number, LCM0211).
Dou R., Nishihara R., Cao Y., Hamada T., Mima K., Masuda A., Masugi Y., Shi Y., Gu M., Li W., da Silva A., Nosho K., Zhang X., Meyerhardt J.A., Giovannucci E.L., Chan A.T., Fuchs C.S., Qian Z.R, & Ogino S. (2016). MicroRNA let-7, T cells, and patient survival in colorectal cancer. Cancer immunology research, 4(11), 927-935.
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8

Isolation of Intestinal Smooth Muscle Cells

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The distal end of the small intestine (terminal ileum) was dissected, embedded in OCT and intestinal SMC were isolated by LCM. Cross-section was immunostained for calponin to identify SMC, and used as a reference for LCM (Suppl.Fig.III). Eight to ten serial sections were stained with Histogene staining solution (Thermo Fisher) as per kit’s instructions. LCM was performed with the Applied Biosystems ArcturusXT LCM System. LCM dissected tissue was used for RNA isolation with PicoPure Frozen RNA Isolation Kit followed by cDNA synthesis with RiboAmp HS Plus cDNA kit (Thermo Fisher) according to the manufacturer’s instructions.
Sukhanov S., Higashi Y., Shai S.Y., Snarski P., Danchuk S., D’Ambra V., Tabony M., Woods T.C., Hou X., Li Z., Ozoe A., Chandrasekar B., Takahashi S.I, & Delafontaine P. (2018). Smooth muscle protein 22 alpha promoter-driven insulin-like growth factor 1 receptor deficiency promotes atherosclerosis. Arteriosclerosis, thrombosis, and vascular biology, 38(10), 2306-2317.
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9

FFPE DNA Extraction for Microdissection

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Formalin‐fixed, paraffin‐embedded (FFPE) specimens were cut at 10 μm, placed on slides, and stored overnight to air dry. The slides were then deparaffinized and stained using a Paradise staining kit (Arcturus™ Paradise™) (#KIT0312S; Thermo Fisher Scientific) in preparation for microdissection. Microdissection was performed using an ArcturusXT™ LCM System (Thermo Fisher Scientific) following the system manual. A PicoPure™ DNA Extraction Kit (#KIT0103; Thermo Fisher Scientific) was used for DNA extraction, and DNA quantification was performed fluorimetrically (Qubit 2.0, #Q32866; Invitrogen).
Hong Y., Limback D., Elsarraj H.S., Harper H., Haines H., Hansford H., Ricci M., Kaufman C., Wedlock E., Xu M., Zhang J., May L., Cusick T., Inciardi M., Redick M., Gatewood J., Winblad O., Aripoli A., Huppe A., Balanoff C., Wagner J.L., Amin A.L., Larson K.E., Ricci L., Tawfik O., Razek H., Meierotto R.O., Madan R., Godwin A.K., Thompson J., Hilsenbeck S.G., Futreal A., Thompson A., Hwang E.S., Fan F, & Behbod F. (2021). Mouse‐INtraDuctal (MIND): an in vivo model for studying the underlying mechanisms of DCIS malignancy. The Journal of Pathology, 256(2), 186-201.
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10

Laser Capture Microdissection Protocol

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Slides were dissected immediately after staining. Histology was categorized by a board-certified pathologist (R.B.W.) according to initial sections stained with hematoxylin and eosin. Cells were dissected on an ArcturusXT LCM System using both the ultraviolet (UV) laser to cut out each sample and the infrared laser to adhere it to a CapSure HS LCM Cap (Thermo Fisher Scientific LCM0215). For bulk samples, roughly 500 cells were captured by area, according to density estimates by cell counting on small areas. For single cells, a cell was dissected from the same area as the corresponding bulk sample, then any additional cells adhering to the cap were ablated with the UV laser. For the ablation validation experiment, two regions of both types (still roughly 500 cells each) were captured on the same cap, and then one region or the other was ablated with the UV laser, except the no-ablation controls (therefore they had roughly 1000 cells). After LCM, the cap was sealed in a 0.5 mL tube (Thermo Fisher Scientific N8010611) and stored at −80°C until library preparation, which was performed within 3 d of dissection.
Foley J.W., Zhu C., Jolivet P., Zhu S.X., Lu P., Meaney M.J, & West R.B. (2019). Gene expression profiling of single cells from archival tissue with laser-capture microdissection and Smart-3SEQ. Genome Research, 29(11), 1816-1825.
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