For both the first and second part, radiographic images were obtained from the coccygeal region from a left‐lateral approach using a fluoroscopic imaging intensifier DHF‐105CX (Hitachi, Tokyo, Japan). In a blinded manner disc height index (DHI) was calculated as previously described40 and normalized to the DHI pre‐transplantation.
Dhf 105cx
Manufactured by Hitachi
Sourced in Japan
The DHF-105CX is a laboratory equipment product manufactured by Hitachi. It functions as a high-speed centrifuge, designed for efficient sample separation and processing in various research and diagnostic applications.
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Lab products found in correlation
5 protocols using dhf 105cx
1
Radiographic Assessment of Disc Height
2
Evaluating Spinal Disc Height Changes
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Plain lumbar X‐ray images were obtained from all canines 2 weeks prior, during, and 4, 8, and 12 weeks after cell transplantation. Images were taken by fluoroscopic imaging intensifier DHF‐105CX (Hitachi, Tokyo, Japan) with 80 kV, 2 mA at a distance of 100 cm. Images were uploaded in OsiriX‐lite (Pixmeo SARL, Bernex, Switzerland) and IVD height and vertebral height were converted to disc height index (DHI) (Figure 1 ) according to published protocols.28 In short, from a medial plane at 3 points, the height of involved IVD and neighboring vertebrae was determined. DHI was determined as . Subsequently, changes in DHI for every assessed disc were calculated over time as the percentage of DHI relative to DHI prior to degeneration induction. Finally, DHI was calculated relative to the internal L6/7 reference control. All data were transferred to Microsoft Excel (Microsoft, Redmond, Washington, USA) and graphically presented by GraphPad Prism (GraphPad Inc., San Diego, California, USA).
3
Radiographic Assessment of Disc Height
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Radiographic assessment was conducted at baseline and 4, 8, and 12 weeks post-operatively. Images were obtained from the coccygeal region with the animals in the supine position using a fluoroscopic imaging intensifier (DHF-105CX, Hitachi, Japan) under 2.5% isoflurane inhalation. In a blinded manner, disc height index (DHI) was calculated and normalized to pre-transplantation DHI [24] (link).
4
Multimodal Monitoring in Respiratory Research
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Heart rate and mean arterial pressure were monitored by using a Philips IntelliVue MP50 Patient Monitor (Philips Medizin Systeme, Bo ¨blingen, Germany). Cardiac output was monitored by using Vigilance II (Edwards Lifesciences). Arterial blood gases were measured by using blood drawn from the femoral artery via an ABL 735 blood gas analyzer (Radiometer Copenhagen, Copenhagen, Denmark). Expiratory tidal volume, airway pressure, and airway flow were recorded by using a CP-100 pulmonary monitor (Bicore, Irvine, California). Radiologic images were taken by using a C-arm (DHF-105CX, Hitachi, Japan). Radiologic findings were assessed from the atelectasis score (0, clear lung fields; 1, plate-like atelectasis or slight infiltration; 2, partial atelectasis; 3, lobar atelectasis; and 4, bilateral lobar atelectasis) 17 and 0-10 cm visual analog scale by 2 investigators blinded to all experimental information.
5
Radiographic Analysis of Tail Deformity
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Frontal radiographs of the tail were taken from all mice under general anesthesia before, 0, 2, and 4 weeks after tail-looping surgery using a fluoroscopic image intensifier (DHF-105CX, Hitachi, Tokyo, Japan). We defined the convex/concave disc height index (DHI) ratio as shown in Fig. 8 F, and measured the Co 8/9 and Co 9/10 levels using Image J in a blinded manner. Changes in the DHI were expressed as %DHI and normalized to the preoperative DHI (%DHI = [postoperative DHI/preoperative DHI] x 100), as described previously [49 (link)].
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