Cells were seeded on glass chamber slides coated with fibronectin for neonatal myocytes and hiPSC-CM, or with laminin for adult myocytes, fixed with 3% formaldehyde / 0.3% triton x-100, then incubated with antibodies (1:100) or phalloidin (in Tris-buffered saline with 1% bovine serum albumin), washed and mounted using Prolong Gold anti-fade with DAPI (Molecular Probes). The antibodies included: anti-OGDH (Cell Signaling Technology, same as those listed for the Western blotting, above), as well as, anti-OGDH (Sigma, cat # HPA019514), and anti-ACAA2 (Origene Technologies, cat # TA506126). The slides were imaged using Nikon A1R laser scanning confocal microscope with Plan Apo 60x objective.
Anti acaa2
Manufactured by OriGene
Anti-ACAA2 is a research antibody product that targets the ACAA2 (acetyl-CoA acyltransferase 2) protein. ACAA2 is an enzyme involved in fatty acid metabolism. This antibody can be used for detection and analysis of the ACAA2 protein in various research applications.
Automatically generated - may contain errors
Lab products found in correlation
Subcellular protein fractionation kit, by Thermo Fisher Scientific (2 mentions)
Anti ogdh, by Cell Signaling Technology (1 mentions)
Anti ogdh, by Merck Group (1 mentions)
Prolong gold antifade with dapi, by Thermo Fisher Scientific (1 mentions)
Anti rna pol 2, by Active Motif (1 mentions)
Criterion gel, by Bio-Rad (1 mentions)
Anti turbogfp, by OriGene (1 mentions)
Odyssey imaging system, by LI COR (1 mentions)
Anti h3, by Active Motif (1 mentions)
Anti fasn, by Abcam (1 mentions)
Anti akt1, by Merck Group (1 mentions)
Vdac1, by GenScript (1 mentions)
Anti h3k9ac, by Active Motif (1 mentions)
2 protocols using anti acaa2
1
Immunofluorescent Imaging of Metabolic Enzymes
2
Recombinant Nucleosomes with Histone Modifications
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Recombinant nucleosomes with modified histones H3K9Ac (cat# 16-0314), H3K9Bu (cat# 16-0371), and H3K18Bu (cat# 16-0373) were purchased from EpiCypher, Inc., and analyzed by Western blotting with the corresponding antibodies.
Cellular protein (25-50 μg) was fractionated using a subcellular protein fractionation kit (Thermo Fisher Scientific, cat# 78,840) according to the manufacturer's protocols. The cellular fractions were separated on a 4%–12% gradient SDS-PAGE (Criterion gels, Bio-Rad) and transferred to nitrocellulose membranes. The antibodies used included anti-H3K9Bu (Abcam, cat# ab241248), anti-H3K18Bu (PTM Bio, cat# PTM-331), anti-H3K9-crotonyl (RevMab, cat# 31-1225,099), anti-H3K27-b-hydroxybutyryl (Abcam, cat# ab241463), anti-H3K9-b-hydroxybutyryl (Thermo Fisher Scientific, PIPA5112503), anti-ACADS (Origene, cat# TA321036), anti-ACAA2 (Origene, cat# TA506126), anti-ACC1/2 (Cell Signaling Technology, cat# 36,765), anti-FASN (Abcam, cat# ab22759), anti-H3 (Active Motif, cat# 61,476), anti-H3K9Ac (Active Motif, cat# 39,917), anti-AKT1 (Millipore 07-416), anti-voltage-dependent anion-selective channel 1 (VDAC1, Genscript, cat# A01419), anti-RNA pol II (Active Motif, cat# 102,660), and anti-turboGFP (Origene, cat# TA150041). Western blotting signals were detected by an Odyssey imaging system (LI-COR) and quantitated using ImageJ.
Cellular protein (25-50 μg) was fractionated using a subcellular protein fractionation kit (Thermo Fisher Scientific, cat# 78,840) according to the manufacturer's protocols. The cellular fractions were separated on a 4%–12% gradient SDS-PAGE (Criterion gels, Bio-Rad) and transferred to nitrocellulose membranes. The antibodies used included anti-H3K9Bu (Abcam, cat# ab241248), anti-H3K18Bu (PTM Bio, cat# PTM-331), anti-H3K9-crotonyl (RevMab, cat# 31-1225,099), anti-H3K27-b-hydroxybutyryl (Abcam, cat# ab241463), anti-H3K9-b-hydroxybutyryl (Thermo Fisher Scientific, PIPA5112503), anti-ACADS (Origene, cat# TA321036), anti-ACAA2 (Origene, cat# TA506126), anti-ACC1/2 (Cell Signaling Technology, cat# 36,765), anti-FASN (Abcam, cat# ab22759), anti-H3 (Active Motif, cat# 61,476), anti-H3K9Ac (Active Motif, cat# 39,917), anti-AKT1 (Millipore 07-416), anti-voltage-dependent anion-selective channel 1 (VDAC1, Genscript, cat# A01419), anti-RNA pol II (Active Motif, cat# 102,660), and anti-turboGFP (Origene, cat# TA150041). Western blotting signals were detected by an Odyssey imaging system (LI-COR) and quantitated using ImageJ.
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