Vs120 digital virtual slide system vs120 l100 w

The H&E- and IHC-stained glass slides were scanned at 40× (0.17 µM/pixel) and digitized to a .vsi format by the Olympus VS120 Digital Virtual Slide System (VS120-L100-W). The files were then converted to a multiresolution tiff format using the quip_converter repository maintained by the Stony Brook Department of Biomedical Informatics ⁶⁴ .
The WSIs are first downsized by a factor of 2, as this resolution is utilized for WSI registration and IHC marker positivity detection, which are covered below. Then, these registered, downsized WSIs are scaled again by a factor of 4 for a total downsized factor of 8. This further downsampling is required for extracted H&E patches to be at the correct resolution to match requirements for the ‘Involved’ versus ‘Uninvolved’ classifier pipeline.

FFPE swiss-rolled colons were sectioned onto glass slides and stained by hematoxylin and eosin (H&E) [16 (link)]. These glass slides were then scanned and digitized at 40X magnification (0.17 μM/pixel) to a.vsi format by the Olympus VS120 Digital Virtual Slide System (VS120-L100-W). The files were converted to a tiff format for further downstream use.