Anaerogen atmosphere generation systems sachets

Tryptic Soy Broth (TSB), deMan, Rogosa, and Sharpe (MRS) Broth and agar, fructose, cysteine-hydrochloride, sodium chloride (NaCl), sodium hydroxide (NaOH), phosphate-buffered saline (PBS), high-glucose Dulbecco’s Modified Eagle Medium (DMEM), trypsin, 4-(2-hydroxyethyl)-1-piperazineethanesulphonic acid (HEPES), methanol, streptomycin–penicillin mixture for cell cultures, trypan blue, mucin from the porcine stomach, acetic acid, ethanol, gelatin, crystal violet, Triton X-100, and n-hexadecane were purchased from Merck Life Science, Warsaw, Poland. Anaerobe Basal Broth (ABB), foetal bovine serum (FBS), GlutaMAX^TM, TrypLE^TM Express, and AnaeroGen^TM Atmosphere Generation Systems sachets were purchased from Thermo Fisher Scientific, Waltham, MA, USA. Cryobanks™ were from Copan Diagnostics Inc., Jefferson Avenue Murrieta, Murrieta, CA, USA. In addition, 6-, 24- and 96-well transparent flat-bottom plates, serological pipettes, and T75 roux flasks (all from Greiner Bio-One GmbH Kremsmünster, Austria) were purchased in Biokom Systems, Janki, Poland. Collagen-coated 96-well plates (BioCoat^®®) were from Becton, Dickinson and Co., Franklin Lakes, NJ, USA. Disposable syringe filters (0.22 µm pore size) were purchased from Labindex S.A., Warsaw, Poland. The Caco-2 cell line was from Cell Line Service GmbH, Eppelheim, Germany.

Tryptic Soy Agar and Broth (TSA and TSB), deMan, Rogosa and Sharpe (MRS) broth and agar, Plate Count Agar (PCA), Malt Extract Agar (MEA), Yeast Extract Glucose Chloramphenicol agar and broth (YGC), Wort Broth and Brain Heart Infusion (BHI) broth, fructose and cysteine-hydrochloride, sodium chloride (NaCl), and sodium hydroxide (NaOH) were purchased from Merck Life Science, Warsaw, Poland. Anaerobe Basal Broth (ABB) and AnaeroGen^TM Atmosphere Generation Systems sachets were purchased from Thermo Fisher Scientific, Waltham, MA, USA. Cryobanks™ were from Copan Diagnostics Inc., Jefferson Avenue Murrieta, Murrieta, CA, USA. In addition, 96-well U-bottom transparent plates were from Greiner Bio-One GmbH Kremsmünster, Austria. Syringe filters (0.22 µm pore size) were purchased from Labindex S.A., Warsaw, Poland.