Cd86 polyclonal antibody

Frozen sections of graft kidneys were thawed at room temperature and washed with PBS. After this, sections were incubated with a blocking solution and then incubated with primary antibodies overnight at 4℃. On the second day, sections were incubated with a secondary antibody and then counterstained with DAPI. An EVOS™ FL Auto 2 confocal microscope (Thermo Fisher) was used to visualize the stained sections. The primary antibodies used in this experiment were as follows: integrin αE antibody (OX62) PE (Santa Cruz Biotechnology, USA), HLA‐DRA polyclonal antibody (Proteintech, USA), and CD86 polyclonal antibody (Proteintech). The secondary antibody used in this experiment was purchased from Boster Biological Technology Co., Ltd. (China).

Dexamethasone was obtained from Beijing Solarbia Technology Co., Ltd. (Beijing, China). IL-6, IL-1β, TNF-α enzyme-linked immunosorbent assay (ELISA) and arginase-1 (Arg-1) ELISA kit was obtained from Shanghai Enzymatic Union Biotechnology Co., Ltd. (Shanghai, China). Inducible nitric oxide synthase (iNOS) and bicinchoninic acid (BCA) protein concentration assay kits were purchased from Nanjing Jiancheng Biological Engineering Institute (Nanjing, China). CD86 polyclonal antibody and CD206 polyclonal antibody were purchased from Proteintech Group, Inc. (Wuhang, China). Poly(I:C) and reference standards of chlorogenic acid, forsythoside A, liquiritin, saikosaponin A, baicalin, arginine, magnolol, protocatechuic acid, 4-hydroxyacetophenone, aristolochic acid A and artemisinin were obtained from Shanghai Yuanye Biotechnology Co., Ltd. (Shanghai, China). All herbs used to prepare QWJR were obtained from Sinopharm Yunnan, Ltd. (Kunming, China).