GLP-1 (7-36), Exendin-4 (EX4; GLP-1R agonist), Exendin 9-39 (Ex9-39; GLP-1R antagonist), para-chlorophenylalanine (PCPA), R-96544 (selective 5HT2A antagonist (21 (link))), SB242084 (5HT2C antagonist (22 (link))), and angiotensin II were purchased from Tocris (Bristol, UK). All substances, except for SB242084, Liraglutide, and PCPA, were dissolved in artificial cerebrospinal fluid (aCSF), vehicle for central injections. Liraglutide (Bachem) was dissolved in 0.9% saline. PCPA was dissolved in 0.9% saline by gentle warming and sonication to a concentration of 100 mg/ml (23 (link), 24 (link)). SB242084 was dissolved in 16% DMSO. 5HT2C receptor antagonist SB242084 displays 158- and 100-fold selectivity over 5HT2A and 5HT2B receptors respectively and it also displays selectivity over a range of other 5-HT, dopamine and adrenergic receptors. R-96544 is a potent, selective 5HT2A receptor antagonist; R-96544 shows 100-fold higher affinity for the human 5HT2A receptors than 5HT1A, 5HT1B, 5HT1D, 5HT5A, 5HT6, 5HT7 receptors and 5-HT transporter, although R-96544 has relatively high affinity for 5HT2C receptors (fourfold less compared to 5-HT2A) (21 (link)).
Para chlorophenylalanine
Manufactured by Bio-Techne
Sourced in United Kingdom
Para-chlorophenylalanine is a chemical compound used for laboratory research purposes. It is a synthetic amino acid analog with a chlorine substituent. This product is commonly utilized in various biological and biochemical applications, but a detailed description of its core function without interpretation or extrapolation cannot be provided.
Automatically generated - may contain errors
2 protocols using para chlorophenylalanine
1
Investigating GLP-1 Receptor Signaling Pathways
2
Effects of PCPA on Social Behavior and Stress in Mice
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The tryptophan hydroxylase inhibitor, parachlorophenylalanine (PCPA) was purchased from Tocris Bioscience. PCPA was dissolved in 0.3 ml 0.9% saline at the dose of 300 mg/kg, and intraperitoneally administered in C57 mice under 2 mpc and 8 mpc. The drug was administered in all mice in each cage. An equivalent volume of saline (SAL) was given to mice as control treatments. Recordings of social interactions in home cages were conducted 24 hours after drug administration, followed by sacrificing animals under 2 mpc and 8 mpc, with blood sampling for CORTS assays. The dose of the drug and timing of behavioral observations after drug administration were determined based on the previous study showing that PCPA at this dose substantially reduced tryptophan hydroxylase expression up to 5 days after administration62 (link)–64 (link).
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