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Cx31 optical microscope

Manufactured by Olympus
Sourced in Japan

The CX31 optical microscope is a compact and durable instrument designed for basic microscopy applications. It features a bright halogen illumination system, a sturdy metal frame, and a range of magnification options up to 1000x. The CX31 is suitable for educational and entry-level laboratory settings.

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18 protocols using cx31 optical microscope

1

Histological Analysis of Corneal Samples

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Control and treated corneas were fixed in 10% formalin. Samples were cut in 5 μm thick sections, embedded in paraffin, stained with hematoxylin and eosin and studied under an Olympus CX31 optical Microscope. Images were collected using the OLYMPUS analySIS getIT software. The same process was followed for the control sample.
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2

Colonic Histological Examination Protocol

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Colonic tissues (1 cm) were removed for histological observation. The tissues were washed in ice-cold normal saline, fixed in 10 % formalin, embedded in paraffin, cut into 4 µm thin sections, and adhered onto plain glass slides. The sections were then baked in an oven at 58 °C for 24 h for dewaxing, stained with hematoxylin and eosin, dehydrated in 95, 90, and 70 % ethanol, cleaned up in xylene, mounted in Permount or Histoclad, and observed at 200× magnification under an Olympus CX31 optical microscope (Olympus, Japan) at 2.80 V and 2.74 A. Images were obtained with a Canon A640 camera (Canon, Japan).
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3

Histological Analysis of Mouse Intestinal Mucosa

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The small intestinal tissue was intercepted 2 cm, fixed by neutral formaldehyde, and paraffin-embedded, and after routine dehydration, hematoxylin-eosin (HE) staining was performed, and then the histological morphology of each layer of the mouse mucosa was observed by CX31 optical microscope (OLYMPUS, Japan). To quantitatively assess intestinal tissue, the average height of small intestinal villi, mean villi area, mean mucosal thickness, and crypt depth were measured using the Taimeng BI2000 image processing system (Chengdu Technology Market Co., Ltd., China).
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4

Histological analysis of coronary tissues

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Coronary tissues were fixed in 10% formalin (NanJing JianCheng Bioengineering Institute, Nanjing, China) for 24 h, followed by dehydration with ethanol (Sigma-Aldrich, St. Louis, Missouri, USA), clearance with xylene, dewaxing, and embedment in paraffin. After that, the paraffin-embedded block was dewaxed and stained with hematoxylin staining solution. Next, the tissues were differentiated by 1% acidic alcohol, immersed in eosin solution, and dehydrated in ethanol. After clearance with xylene twice (10 min), the tissues were covered with Acacia senegal and observed under a CX-31 optical microscope (Olympus, Tokyo, Japan). An LM1235 ultra-thin semi-automatic slicer (Leica, Wetzlar, Germany) was utilized for sectioning while a KD-BM machine for tissue embedment (Kedee, Zhejiang, China).
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5

Histological Tissue Analysis Protocol

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After ex vivo studies, each tissue was fixed in buffered 10% formalin and embedded in paraffin wax. Four-micrometer-thick sections of the tissue were stained with hematoxylin-eosin and were placed on microscope slides. The tissue sections were analyzed with an Olympus CX31 optical microscope and images were processed with OLYMPUS analysis getIT software (Version 5-1, Shinjuku-ku, Tokyo, Japan).
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6

Histopathological Assessment of Fish Tissues

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Of the 15 fish, remaining 6 were used for histological assessment. The gill, liver and kidney tissues of the fish sampled on days 0, 7 and 14 were examined histopathologically. After fixing in Bouin’s solution for 8 h, the tissues were stored in 70% ethanol until the preparation of wax blocks. During the preparation stage, the tissues were passed through 80%, 90% and 96% absolute ethanol, respectively. Tissues were then passed through xylene and embedded in paraffin. Five µm cross-sections were cut from the paraffin blocks and stained with hematoxylin and eosin (H&E) for routine wax histology. Slides were examined under an Olympus CX31 optical microscope. Photographs were captured by a camera attached to an Olympus BX51 optical microscope using Olympus Analysis LS software.
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7

Optical Characterization of MAP and MCMAP

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Optical micrographs of MAP and MCMAP were obtained using an Olympus CX 31 optical microscope attached to a computer system. Samples were placed on glass slides, and a few drops of ethanol were added to disperse the powders. A cover glass was then placed on top of each suspension, and the micrographs were captured using a computer system.
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8

Optical Observation of CCS Microgranules

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The optical observations of the CCS microgranules were carried out using an Olympus CX31 optical microscope (Cebu, Philippines) under 100-time magnification. The samples were prepared by dyeing cationic starch derivative microgranules with an 0.005 M standard iodine solution. The photographs of the CCS slurry were taken using an Olympus camera.
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9

Anther Sectioning and Staining Protocol

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Flower buds with different lengths were fixed in formalin-aceto-alcohol (FAA), dehydrated in an ethanol series, embedded in paraffin, sectioned into 3–5 μm transverse slices using a microtome and stained with 1% toluidine blue as described by Lou et al. [40 (link)]. Then the anther transverse sections were observed with an Olympus CX31 optical microscope (Olympus Japan Co., Tokyo, Japan) and photographed with a Nikon 550D camera (Canon, Tokyo, Japan).
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10

Leaf Histochemistry of Drymis brasiliensis

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Histochemical tests were carried out in Laboratório de Anatomia Vegetal of Departamento de Biologia from Universidade Federal de Lavras, Brazil, according to the methodology proposed by Figueiredo et al. ([2007 ]). The tests were applied on 3 leaves/individual of D. brasiliensis, being 4 individuals/altitude level (1900 and 2100 m). The cross sections were carried out from approximately 2 cm2 fragments, taken from the region that contains the central vein with the help of an LPC bench microtome.
For the detection of total lipids, the reagent Sudan IV was used; for the detection of the essential oils, the reagent NADI was used, and for the verification of the phenolic compounds, ferric chloride was used. The sections were mounted on slides and coverslips with 50% glycerin, and photographed in an Olympus CX 31 optical microscope, coupled to a digital camera.
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