Rabbit anti s6 ribosomal protein

Manufactured by Cell Signaling Technology

Sourced in United States

Rabbit anti-S6 ribosomal protein is a primary antibody that recognizes the S6 ribosomal protein. The S6 ribosomal protein is a component of the 40S subunit of the eukaryotic ribosome and plays a role in protein synthesis.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

S6 ribosomal protein (94 citations) Anti-S6 (87 citations) Anti-S6 ribosomal protein (26 citations) Rabbit anti-S6 (10 citations)

6 protocols using rabbit anti s6 ribosomal protein

Antibody Characterization Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following antibodies were obtained from commercial sources: rabbit anti-green fluorescent protein (GFP; catalog no. 598, Medical and Biological Laboratories, Woburn, MA, USA), rabbit anti-S6 ribosomal protein (catalog no. 2217, Cell Signaling Technology, Danvers, MA, USA), rabbit anti-phospho-S6 (Ser-240/244, catalog no. 2215, Cell Signaling Technology), rabbit anti-phospho-Akt (Ser-473, catalog no. 4060, Cell Signaling Technology), mouse anti-Akt (catalog no. 2920, Cell Signaling Technology), mouse anti-β-galactosidase (catalog no. Z3781, Promega, Madison, WI, USA), rabbit anti-β-galactosidase (catalog no. 559762, MP Biomedicals, Santa Ana, CA, USA), mouse anti-monoubiquitinylated and polyubiquitinylated conjugates (clone FK; catalog no. BML-PW8810, Enzo Life Sciences, Farmingdale, NY, USA), and mouse anti-tubulin (catalog no. T5168, Sigma-Aldrich, St. Louis, MO, USA). Anti-mouse and anti-rabbit secondary antibodies conjugated to Alexa Fluor 488 or Alexa Fluor 647 were obtained from Invitrogen (Carlsbad, CA, USA). IRDye-conjugated secondary donkey anti-mouse IRDye 680RD antibody (catalog no. 926-68072) and donkey anti-rabbit IRDye 800CW antibody (catalog no. 926-32213) were obtained from LI-COR Biosciences (Lincoln, NE, USA). Insulin was obtained from Sigma-Aldrich (St. Louis, MO, USA).

Firkowska M., Macias M, & Jaworski J. (2018). ESCRT Proteins Control the Dendritic Morphology of Developing and Mature Hippocampal Neurons. Molecular Neurobiology, 56(7), 4866-4879.

+ Open protocol

+ Expand

Protein Extraction and Immunoblotting Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Snap frozen embryos or brains for protein extraction were minced in extraction buffer (150 mM NaCl, 1% NP-40, 0.5% deoxycholate, 0.1%SDS, 50 mM Tris-HCl pH 7.5) and incubated at 4 °C for 30 minutes. Lysates were separated on Invitrogen Bolt precast 4–12% polyacrylamide gels and transferred to PVDF membrane before blotting. Antibodies and their corresponding dilutions were: rabbit anti phospho-S6 Ribosomal Protein (Ser235/236) (Cell Signalling, #2211, 1:1000) rabbit anti phospho-S6 Ribosomal Protein (Ser240/244) (Cell Signalling, #5364, 1:1000), rabbit anti phospho-p70 S6 Kinase (Thr389) (Cell Signalling, #9234, 1:1000), rabbit anti phospho-AKT (Ser473) (Cell Signalling, #4060, 1:2000), rabbit anti phospho-AKT (Thr308) (Cell Signalling, #13038, 1:750), rabbit anti p70 S6 Kinase (Cell Signalling, #9202, 1:500), rabbit anti S6 Ribosomal Protein (Cell Signalling, #2217, 1:750) and rabbit anti mTOR (Cell Signalling, #2983, 1:1000).

Hughes J., Dawson R., Tea M., McAninch D., Piltz S., Jackson D., Stewart L., Ricos M.G., Dibbens L.M., Harvey N.L, & Thomas P. (2017). Knockout of the epilepsy gene Depdc5 in mice causes severe embryonic dysmorphology with hyperactivity of mTORC1 signalling. Scientific Reports, 7, 12618.

+ Open protocol

+ Expand

Phospho-Protein Analysis in BCPAP Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Whole cell lysates were isolated using standard RIPA buffer containing proteases and phosphatases and quantified using the Lowery protein assay (BioRad). 50 μg of lysates from BCPAP cells treated with DMSO vehicle or increasing concentrations (25 nM, 50 nM, or 100 nM) of TM for 7 days or TM (EC_6.25, 25 μM), vemurafenib (EC_6.25, 6.25 μM) or both drugs at the same concentrations for 7 days were resolved by SDS-PAGE and immunoblotted with a rabbit anti-phospho(Thr 202/Tyr 204)-ERK1/2 antibody (Cell Signaling Technology, antibody # 3700 at a 1:1000 dilution), a mouse anti-ERK1/2 antibody (Cell Signaling Technology, antibody # 9101 at a 1:1000 dilution), a mouse anti-HA-Tag antibody (Cell Signaling Technology, antibody # 2367 at a 1:1000 dilution), a rabbit anti-phospho-S6 ribosomal protein (Ser235/236) antibody (Cell Signaling Technology, antibody #4858S at a 1:1000 dilution), a rabbit anti-S6 ribosomal protein (Cell Signaling Technology, antibody #2217 at a 1:1000 dilution), or a mouse anti-β-tubulin (Sigma-Aldrich, antibody # 2367 at a 1:5000 dilution) followed by a goat anti-rabbit IgG (Cell Signaling Technology, antibody # 7076) or a goat anti-mouse IgG (Cell Signaling Technology, antibody # 7074) horseradish peroxidase-conjugated secondary antibody and visualized using enhanced chemiluminescence detection (Cell Signaling Technology).

Xu M., Casio M., Range D.E., Sosa J.A, & Counter C.M. (2018). Copper chelation as targeted therapy in a mouse model of oncogenic BRAF-driven papillary thyroid cancer. Clinical cancer research : an official journal of the American Association for Cancer Research, 24(17), 4271-4281.

+ Open protocol

+ Expand

Western Blot Analysis of Cell Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Protein lysates were obtained from cells or excised tumors from transplanted mice through solubilization in lysis buffer [PBS; 1% Sodium Dodecyl Sulfate (SDS; Sigma-Aldrich 05030); PhosSTOP™ (Roche 04 906 845 001); cOmplete™ Protease Inhibitor Cocktail (Roche 11873580001)], separated on 8–15% SDS-PAGE in reducing conditions and transferred onto nitrocellulose membrane (GE Healthcare 10600002). Western blot (WB) analyses were performed following standard protocols. The following Abs were used: rat ant-IRF4 (1:500; Biolegend, 646402); rabbit anti-BLIMP1 (1:1000; Cell Signaling, 9115); rabbit anti-phospho eIF2α (1:1000; Cell Signaling, 3398); rabbit anti-eIF2α tot (1:1000; Cell Signaling, 5324); rabbit anti-phospho S6 Ribosomal Protein (1000; Cell Signaling, 5364); rabbit anti-S6 Ribosomal Protein (1000; Cell Signaling, 2317); rabbit anti-LC3B polyclonal Ab (1:500; Novus Biologicals, NB100–220); rabbit anti-phospho AKT (S473) (1:1000; Cell Signaling, 9271); rabbit anti-phospho AKT (T308) (1:1000; Cell Signaling, 2965) rabbit anti-Akt (pan) (C67E7) (1:1000; Cell Signaling, 4691); rabbit anti-phospho BAD (1:1000; Cell Signaling, 4366); rabbit anti-BAD tot (1:1000; Cell Signaling, 9239); mouse anti-ACTB/b actin (1:5000; Sigma-Aldrich, A5441). Quantifications were obtained with the gel analysis option of ImageJ software.

Trudu M., Oliva L., Orfanelli U., Romano A., Di Raimondo F., Sanvito F., Ponzoni M, & Cenci S. (2022). Preclinical evidence of a direct pro-survival role of arginine deprivation in multiple myeloma. Frontiers in Oncology, 12, 968208.

+ Open protocol

+ Expand

Western Blot Analysis of Cell Signaling Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells in microslides were washed twice with ice-cold PBS and lysed on ice with 150 μl of 1X Laemmli buffer (60 mM Tris-HCl pH=6.8, 2% SDS, 10% Glycerol, bromophenol blue, supplemented with 100 mM DTT) for 30 min. Samples were boiled for 10 min at 95°C, separated by SDS/PAGE and then transferred onto Nitrocellulose membranes. Western blot analysis was performed with specific antibodies and the antigen–antibody complexes were visualized by chemiluminescence (Immobilon Western, Merck Millipore). The following antibodies were used in immunoblotting: rabbit-anti LC3 (Sigma, Cat#L7543); rabbit-anti-FLCN (Cell signaling, Cat#3697); rabbit-anti-FNIP1 (Abcam, Cat#ab134969); rabbit-anti-AMPK (Cell signaling, Cat#2532S); rabbit-anti-p-AMPK (T172) (Cell signaling, Cat#2535); mouse-anti-actin (Millipore, Cat#1501); rabbit-anti-ATG16L1 (MBL, Cat#PM040); rabbit-anti-IFT20 (Proteintech, Cat#13615-1-AP); rabbit-anti β-catenin (Cell signaling, Cat#8480); rabbit-anti-LKB1 (Cell signaling, Cat#3050); rabbit-anti-S6 ribosomal protein (Cell signaling, Cat#2217); rabbit-anti-p-S6 ribosomal protein (S240/244) (Cell signaling, Cat#2215); rabbit-anti-Tuberin/TSC2 (Cell signaling, Cat#4308); rabbit-anti-p-Tuberin/TSC2 (T1462) (Cell signaling, Cat#3617). Secondary HRP conjugate anti-rabbit IgG (GE Healthcare) and HRP conjugate anti-mouse IgG (Bio-Rad).

Zemirli N., Boukhalfa A., Dupont N., Botti J., Codogno P, & Morel E. (2019). The primary cilium protein folliculin is part of the autophagy signaling pathway to regulate epithelial cell size in response to fluid flow. Cell Stress, 3(3), 100-109.

+ Open protocol

+ Expand

Subcellular Fractionation and Western Blot Analysis of HGPS iPSC-Osteoprogenitors

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Western blots were performed as previously described.^{(35 (link))} Subcellular fractions of normal and HGPS iPSC-osteoprogenitors were obtained by NE-PER Nuclear and Cytoplasmic Extraction Kit (#78835; Bio-Rad), according to manufacturer’s directions. For long bone extracts, tibiae were dissected from both genotypes and cleaned of soft tissue. Subsequently, the epiphyses were removed, the marrow cavity flushed with sterile saline, prior to lysis, as described.^{(34 )(36 (link))} Primary antibodies used for immunoblotting analysis are as follows: rabbit anti-non-phosphorylated (Active) β-catenin protein Ser33/37/Thr41 (#4270; Cell Signaling Technology); mouse anti-lamin A/C (MAB3211; EMD Millipore); goat anti-lamin A/C N-18 (sc-6215; Santa Cruz Biotechnology); rabbit anti-RCC1 (#3589; Cell Signaling Technology); rabbit anti-S6 ribosomal protein (#2217; Cell Signaling Technology); monoclonal anti-β-Actin-peroxidase (#A3854; Sigma-Aldrich). Protein densitometry was analyzed by using Image lab software to eliminate the saturation of band and quantify normalized protein expression values.

Choi J.Y., Lai J.K., Xiong Z.M., Ren M., Moorer M.C., Stains J.P, & Cao K. (2018). Diminished canonical β-catenin signaling during osteoblast differentiation contributes to osteopenia in progeria. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 33(11), 2059-2070.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!