Gm130 610822

Manufactured by BD

The GM130 (610822) is a laboratory equipment designed for cell culture applications. It functions as a Golgi matrix protein that is involved in the structure and organization of the Golgi apparatus within eukaryotic cells.

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Lab products found in correlation

4 6 diamidino 2 phenylindole dapi d1306, by Thermo Fisher Scientific (1 mentions) Gapdh sc 25778, by Santa Cruz Biotechnology (1 mentions) Tom20 sc 11415, by Santa Cruz Biotechnology (1 mentions) Asc ag 25b 0006, by Adipogen (1 mentions) Ha tag 6e2, by Cell Signaling Technology (1 mentions) Alexa fluor dye conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions) Myc tag 9b11, by Cell Signaling Technology (1 mentions)

3 protocols using gm130 610822

Comprehensive Immunohistochemical Analysis

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Antibodies used in the study are: αSMA (ab7817) and Ki67 (ab15580) from Abcam; GM130 (610822) from BD Biosciences; cleaved caspase-3 (9664S) and PDGFRα (3174S) from Cell Signaling Technology; BrdU (GTX26326) from GeneTex; pro-surfactant protein C (10774–1-AP) from Proteintech; caveolin-1 (sc-894), Hopx (sc-398,703) and GAPDH (sc25778) from Santa Cruz Biotechnology; β-actin (A5441) and α-tubulin (T5168) from Sigma-Aldrich; and AlexaFluor 488, 594 or 647-conjugated secondary antibodies from Invitrogen. Chemicals used in the study are: ProLong antifade (P36930) and 4′,6-diamidino-2-phenylindole (DAPI, D1306) reagents from Invitrogen; and Avertin (T48402), methacholine (A2251) and 5-bromo-2′-deoxyuridine (B9285) from Sigma-Aldrich.

Lai Y.T., Chao H.W., Lai A.C., Lin S.H., Chang Y.J, & Huang Y.S. (2020). CPEB2-activated PDGFRα mRNA translation contributes to myofibroblast proliferation and pulmonary alveologenesis. Journal of Biomedical Science, 27, 52.

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Antibody Reagents for NLRP3 Inflammasome Analysis

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Antibodies against NLRP3 (AG-20B-0014) and ASC (AG-25B-0006) were from AdipoGen. Antibodies against caspase-1 (sc-515), human TGN38 (sc-33783) and TOM20 (sc-11415) were from Santa Cruz Biotechnology. Antibody against murine TGN38 was a kind gift from Dr. Matthew Seaman (Cambridge Institute for Medical Research). Antibodies against GOLGA4 (611280) and GM130 (610822) were from BD Biosciences. Antibodies against tubulin (T5168), Flag (F1804) and AP1G1 (A4200) were from Sigma. Antibody against HA (mms-101p) was from Covance. Antibody against giantin (ab24586) was from Abcam. Antibody against COX IV (20E8C12) was from Invitrogen. Antibodies against calreticulin (#2891) and GAPDH (#2118) were from Cell Signaling. Antibody against IL-1β (AB-401-NA) was from R&D Systems. Antibody against ERGIC-53 (ALX-804-602-C100) was from Axxora. Alexa Fluor secondary antibodies (488, 568, and 633) were from Life Technologies.
Flag antibody-conjugated agarose (A2220), nigericin (N7143) and ATP (A7699) were from Sigma. Gramicidin (ALX-350-233) was from Enzo life sciences. Rapamycin (AG-CN2-0025) was from AdipoGen. LPS (Ultra-Pure), imiquimod (tlrl-imq) and CL097 (tlrl-c97-5) were from InvivoGen.

Chen J, & Chen Z.J. (2018). PtdIns4P on Dispersed Trans-Golgi Network Mediates NLRP3 Inflammasome Activation. Nature, 564(7734), 71-76.

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Immunoblotting Antibody Validation Protocol

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Caveolin-1 (pAB #610060, 2234 #610494 and 2297 #610406), caveolin-2 (#610684) and GM130 (#610822) antibodies were purchased from BD Biosciences (Table 2). Caveolin-1 C-term [E249] (ab32577), PTRF/Cavin1 (ab48824) and EHD2 (ab23935) antibodies were purchased from Abcam. The PACSIN2 (AP088b) antibody used was purchased from Abgent. Alexa-fluor dye-conjugated secondary antibodies were purchased from Life Technologies. A c-Myc (A-14) antibody was purchased from Santa Cruz Biotechnology. HA-Tag (6E2) and Myc-Tag (9B11) antibodies used were purchased from Cell Signaling Technology.

Han B., Copeland C.A., Kawano Y., Rosenzweig E.B., Austin E.D., Shahmirzadi L., Tang S., Raghunathan K., Chung W.K, & Kenworthy A.K. (2016). Characterization of a caveolin-1 mutation associated with both pulmonary arterial hypertension and congenital generalized lipodystrophy. Traffic (Copenhagen, Denmark), 17(12), 1297-1312.

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