Mouse anti α sma

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Mouse anti-α-SMA is an antibody that recognizes the alpha-smooth muscle actin (α-SMA) protein. α-SMA is a cytoskeletal protein found in smooth muscle cells and is commonly used as a marker for these cell types.

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5 protocols using mouse anti α sma

Isolation and Analysis of Coronary Arteries

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Freshly isolated coronary arteries were obtained from consented heart transplant recipients or heart donors as described^{39 (link),109}. Briefly, hearts were arrested in cardioplegic solution, transferred on ice, and left anterior descending (LAD), left circumflex artery and right coronary arteries were dissected from the epicardium, with surrounding adipose and myocardial tissue carefully removed. Coronary artery segments were grossly scored for presence of lesions, embedded in OCT, snap frozen in liquid nitrogen, and stored at −80 °C until analysis. OCT blocks were cryosectioned at 8 μm. Frozen sections were washed, fixed in 4% formaldehyde, permeabilized with triton X-100 at 0.05%, blocked with donkey serum and incubated overnight with primary antibodies as follows: mouse anti-ENPP1 (SC-166649, Santa Cruz Biotechnology, Dallas, TX), rabbit anti-IGFBP3 (#10189–2-AP, Proteintech, Rosemont, IL), rabbit anti-ARID5B (#HPA015037, Atlas Antibodies, Stockholm, SWE), or mouse anti-ADK (SC-514588, Santa Cruz Biotechnology) at 1:100 dilution and mouse anti-α-SMA (SC-53142, Santa Cruz Biotechnology) at 1:100 dilution. Slides were washed with PBS-Tween and incubated with appropriate secondary antibodies. More detailed methods are included in the Supplementary Note.

Kavousi M., Bos M.M., Barnes H.J., Lino Cardenas C.L., Wong D., Lu H., Hodonsky C.J., Landsmeer L.P., Turner A.W., Kho M., Hasbani N.R., de Vries P.S., Bowden D.W., Chopade S., Deelen J., Benavente E.D., Guo X., Hofer E., Hwang S.J., Lutz S.M., Lyytikäinen L.P., Slenders L., Smith A.V., Stanislawski M.A., van Setten J., Wong Q., Yanek L.R., Becker D.M., Beekman M., Budoff M.J., Feitosa M.F., Finan C., Hilliard A.T., Kardia S.L., Kovacic J.C., Kral B.G., Langefeld C.D., Launer L.J., Malik S., Mohamed Hoesein F.A., Mokry M., Schmidt R., Smith J.A., Taylor K.D., Terry J.G., van der Grond J., van Meurs J., Vliegenthart R., Xu J., Young K.A., Zilhao N.R., Zweiker R., Assimes T.L., Becker L.C., Bos D., Carr J.J., Cupples L.A., de Kleijn D.P., de Winther M., den Ruijter H.M., Fornage M., Freedman B.I., Gudnason V., Hingorani A.D., Hokanson J.E., Arfan Ikram M., Isgum I., Jacobs DR J.r., Kähonen M., Lange L.A., Lehtimäki T., Pasterkamp G., Raitakari O., Schmidt H., Eline Slagboom P., Uitterlinden A.G., Vernooij M.W., Bis J.C., Franceschini N., Psaty B.M., Post W.S., Rotter J.I., Björkegren J.L., O’Donnell C.J., Bielak L.F., Peyser P.A., Malhotra R., van der Laan S.W, & Miller C.L. (2023). Multi-ancestry genome-wide study identifies effector genes and druggable pathways for coronary artery calcification. Nature genetics, 55(10), 1651-1664.

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Comprehensive Antibody Panel for Fibrosis Analysis

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The following antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA): goat anti-COL1A1, anti-p-p70s6k (Thr389), and anti-Akt; mouse anti-α-SMA, anti-LC-3, anti-mTOR, and anti-GNMT; rat anti-CD3; and rabbit anti-p70s6k, anti-p62, anti-p-mTOR (Ser2448), and anti-COL4A2. Mouse anti-p-Akt (Ser473) and rabbit ant-iNOS and anti-MPO antibodies were obtained from Cell Signaling Technology (Beverly, MA, USA). Rat anti-F4/80 and rabbit anti-ICAM-1 and anti-VCAM-1 antibodies were obtained from Abcam (Cambridge, MA, USA). Both the mouse anti-GAPDH antibody and Masson’s trichrome staining kit were obtained from Sigma–Aldrich (St. Louis, MO, USA). The mouse anti-TGF-β antibody was obtained from R&D Systems (Minneapolis, MN, USA).

Kuo K.L., Chiang C.W., Chen Y.M., Yu C.C, & Lee T.S. (2023). Folic Acid Ameliorates Renal Injury in Experimental Obstructive Nephropathy: Role of Glycine N-Methyltransferase. International Journal of Molecular Sciences, 24(7), 6859.

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Immunofluorescence Staining of Cellular Markers

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Cells were seeded at a density of 20 000 cells/well on coverslips in 24‐well plates. After the indicated stimulation for 24‐72 hours, cells were washed in ice‐cold PBS, fixed in 4% paraformaldehyde in PBS and stained as described before.22 In short, cells were washed with PBS and incubated for 30 minutes in 0.1% Triton X‐100% and 0.5% BSA in PBS for blocking and permeabilization. Subsequently, cells were incubated with the following primary antibodies: rabbit‐anti‐ZO‐1 (1:200; Thermo Fisher; 617300) or mouse‐anti‐α‐SMA (1:1000; Santa Cruz; sc‐32251). After overnight incubation, cells were washed with PBS and incubated with Alexa488‐linked secondary anti‐mouse or anti‐rabbit antibodies for 1 hour. The cytoskeleton was stained using phalloidin (100 nM in PBS, 30 minutes) and nuclei were stained with DAPI (10 minutes). Coverslips were mounted onto object glass slides using prolong gold antifade reagent (Thermo Fisher). Micrographs were made using a Leica DM5000B fluorescent microscope with LAS X software (Leica).

Waasdorp M., de Rooij D.M., Florquin S., Duitman J, & Spek C.A. (2018). Protease‐activated receptor‐1 contributes to renal injury and interstitial fibrosis during chronic obstructive nephropathy. Journal of Cellular and Molecular Medicine, 23(2), 1268-1279.

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Western Blot Analysis of Cellular Signaling

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MCF were harvested and lysed in a lysis buffer. Western blots were performed as previously described [14 (link)]. Equal amount of proteins from each sample were subjected to SDS-PAGE, and then transferred to nitrocellulose membranes. The membranes were blocked with 5 % BSA and incubated at 4 °C overnight with specific primary antibodies: rabbit anti-PCNA (diluted 1:100), rabbit His-tag (diluted 1:100), goat anti-collagen I (diluted 1:200), mouse anti-α-SMA (diluted 1:100), rabbit anti-TGF-β1 (diluted 1:200), goat anti-gp91^phox (diluted 1:200) (Santa Cruz Biotechnology, USA), or mouse anti-collagen III (diluted 1:200) (Sigma, USA). The horseradish peroxidase-conjugated second antibodies (diluted 1:5000) (Zhongshan Biotechnology, China) were incubated for 2 h at room temperature. Proteins were detected by ECL detection system.

Tan L.G., Xiao J.H., Yu D.L., Zhang L., Zheng F., Guo L.Y., Yang J.Y., Tang J.M., Chen S.Y, & Wang J.N. (2015). PEP-1-SOD1 fusion proteins block cardiac myofibroblast activation and angiotensin II-induced collagen production. BMC Cardiovascular Disorders, 15, 116.

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Immunofluorescence Staining of Fibroblasts

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Fibroblasts were seeded in 48-well plates at 6060 cells/cm² and, after overnight attachment, cells were treated with drugs as described above. After 1 and 4 d, cells were fixed with paraformaldehyde and stained for immunofluorescence using primary mouse anti-α-SMA (Santa Cruz Biotechnology, Dallas, TX, USA, sc-32251) and secondary goat anti-mouse IgG AlexaFluor488-conjugated antibodies (Thermo Fisher Scientific A-11001), and DNA was counterstained with Hoechst 33342 as previously described [50 (link)].

Weathers P., Towler M., Kiani B.H., Dolivo D, & Dominko T. (2024). Differential Anti-Fibrotic and Remodeling Responses of Human Dermal Fibroblasts to Artemisia sp., Artemisinin, and Its Derivatives. Molecules, 29(9), 2107.

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