The following antibodies and lectins were used: anti-myc (mouse, clone 4A6; Millipore; catalog no.: 05-724), anti-BACE1 (mouse, clone 1A11, generous gift from Dr Bart De Strooper (40 (link))), anti-GAPDH (mouse, clone 6C5; Merck Millipore; catalog no.: MAB374), anti-Golgin-97 (rabbit, clone D8P2K, Cell Signaling Technology; catalog no.: 13192S), anticalnexin (rabbit, abcam; catalog no.: ab22595), anti-N-cadherin (rabbit, abcam; catalog no.: ab18203), horseradish peroxidase (HRP)–conjugated antimouse IgG (GE Healthcare; catalog no.: NA931V), HRP-conjugated anti-rabbit IgG (GE Healthcare, Amersham; catalog no.: NA934V), DSA (J-Chemical; catalog no.: J105), SSA letin (J-Chemical; catalog no.: J118), biotinylated RCA (Vector Laboratories; catalog no.: B-1085), Alexa546-conjugated anti-rabbit IgG (Invitrogen; catalog no.: A10040), and Alexa488-conjugated antimouse IgG (Invitrogen; catalog no.: A21202). DSA and SSA were conjugated to HRP using a Peroxidase Labeling Kit-NH2 (Dojindo), according to the manufacturer’s protocol.
Biotinylated rca
Manufactured by Vector Laboratories
Biotinylated RCA is a horseradish peroxidase-labeled lectin product from Vector Laboratories. It is used as a molecular biology tool for detecting and localizing carbohydrates and glycoproteins in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Hrp conjugated anti rabbit igg, by GE Healthcare (2 mentions)
Horseradish peroxidase hrp conjugated anti mouse igg, by GE Healthcare (2 mentions)
Anti golgin97, by Cell Signaling Technology (2 mentions)
Peroxidase labeling kit nh2, by Dojindo Laboratories (2 mentions)
Anti gapdh, by Merck Group (2 mentions)
Alexa546 conjugated anti rabbit igg, by Thermo Fisher Scientific (1 mentions)
Anti myc, by Merck Group (1 mentions)
Alexa 488 conjugated anti mouse igg, by Thermo Fisher Scientific (1 mentions)
Anti n cadherin, by Abcam (1 mentions)
Anti calnexin, by Abcam (1 mentions)
Anti lamp1, by Abcam (1 mentions)
Anti rab5, by Cell Signaling Technology (1 mentions)
Alexa 488 conjugated anti rabbit igg, by Thermo Fisher Scientific (1 mentions)
Anti rab11, by Cell Signaling Technology (1 mentions)
Anti grp78 bip, by Abcam (1 mentions)
Anti rab7, by Cell Signaling Technology (1 mentions)
Alexa546 conjugated anti mouse igg, by Thermo Fisher Scientific (1 mentions)
Anti gm130, by Cell Signaling Technology (1 mentions)
2 protocols using biotinylated rca
1
Antibodies and Lectins for Cell Analysis
2
Antibody and Lectin Toolkit for Cell Biology
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The following antibodies and lectins were used: anti-GnT-V (mouse, clone 706824, R&D Systems), anti-α1 sodium potassium ATPase (mouse, clone 464.6, Abcam), anti-LAMP1 (rabbit, Abcam), anti-GAPDH (mouse, clone 6C5, Merck Millipore), anti-GRP78 (BiP) (rabbit, Abcam), anti-GM130 (rabbit, clone D6B1) (Cell Signaling Technology), anti-Golgin97 (rabbit, clone D8P2K, Cell Signaling Technology), anti-Rab5 (rabbit, clone C8B1, Cell Signaling Technology), anti-Rab7 (rabbit, Cell Signaling Technology), anti-Rab11 (rabbit, Cell Signaling Technology), horseradish peroxidase (HRP)-conjugated anti-mouse IgG (GE Healthcare), HRP-conjugated anti-rabbit IgG (GE Healthcare), unconjugated-L4-PHA (J-Chemical), biotinylated-SSA (J-Chemical), biotinylated-RCA (Vector Laboratories), FITC-conjugated L4-PHA (J-Oil Mills), Rhodamine-conjugated L4-PHA (Vector Laboratories), Alexa546-conjugated anti-mouse IgG (Invitrogen), Alexa488-conjugated anti-rabbit IgG (Invitrogen). L4-PHA was conjugated to HRP using a Peroxidase Labeling Kit-NH2 (Dojindo) as described previously (22 (link)).
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