C57BL/6 (B6) mice were purchased from Taconic Farms (Hudson, NY). B6.SJL (CD45.1/1), B6.SJL/C57BL/6 (CD45.1/2) mice were obtained through a supply contract between the National Institute of Allergy and Infectious Diseases (NIAID) and Taconic Farms. Epx-Cre mice (Doyle et al., 2013 (link)) were initially obtained from Elizabeth Jacobsen (Mayo Clinic, Phoenix, AZ) and subsequently crossed to ROSA26-LSL-tdTomato mice to generate eosinophil-specific fluorescent EPX-reporter animals and generously gifted by David Sacks (NIAID) (Lee et al., 2020b (link)). Gpr183−/− mice (Baptista et al., 2019 (link); Liu et al., 2011 (link)) were gifted by Michael Fessler (NIEHS) and Vanja Lazarevic (NCI). Ccr3−/− (JAX 5440, C.129S4-Ccr3tm1Cge/J) on the BALB/c background, BALB/c (JAX 651), BALB/c CD45.1 (JAX 6584, CByJ.SJL(B6)-Ptprca/J) and Ch25h−/− (JAX 16263, B6.129S-Ch25htm1Rus/J) mice were purchased from Jackson Laboratories (Bar Harbor, ME). Both male and female mice, 8–24 wk. old at onset of experiments were used and experimental groups in individual experiments were age and sex matched. All animals were bred and maintained in an AAALAC-accredited ABSL2 or ABSL3 facility at the NIH and experiments performed in compliance with an animal study proposal approved by the NIAID Animal Care and Use Committee (protocol LCIM17E).
C57bl 6 b6 mice
Manufactured by Taconic Biosciences
Sourced in Montenegro
C57BL/6 (B6) mice are a widely used inbred mouse strain. They are a common laboratory mouse model and are known for their robust health, ease of breeding, and suitability for a variety of research applications.
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9 protocols using c57bl 6 b6 mice
1
Generation and Characterization of Transgenic Mice
2
Murine Models for Neurological Research
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Male C57BL/6 (B6) mice and Rag2−/−γc−/− mice were purchased from Taconic (Taconic Biosciences). The mutant mice were back-crossed to the B6 background for 12 generations. For all experiments, 6 - to 12-week-old, 23–25 g body weight, age-matched littermates were used. All mice were randomly assigned to experimental groups. Randomization was based on the random number generator function in Microsoft Excel 2013. Mice were housed under standardized light-dark cycle conditions with access to food and water ad libitum. All surgeries were performed under isoflurane anesthesia. Mice were housed in pathogen-free conditions at the animal facilities. All animal experiments were performed in accordance with the recommendations of the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health and in accordance with the ARRIVE (Animal Research: Reporting In Vivo Experiments) guidelines. Animal studies were approved by the Animal Care and Use Committees of the Barrow Neurological Institute and Tianjin Neurological Institute.
3
Experimental Autoimmune Encephalomyelitis in Mice
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Male C57BL/6 (B6) mice and Rag2−/− mice (two- to three-month-old, 23–25 g body weight) were purchased from Taconic (Taconic Biosciences). The mutant mice were back-crossed to the B6 background for 8–12 generations. Mice were housed in pathogen-free conditions at the animal facilities of the Barrow Neurological Institute, St. Joseph's Hospital and Medical Center (Phoenix, AZ) and the Tianjin Neurological Institute, Tianjin Medical University General Hospital (Tianjin, China). All animal experiments were performed in strict accordance with the recommendations of the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health and in accordance with the ARRIVE (Animal Research: Reporting in vivo Experiments) guidelines. The protocol was approved by the Committee on the Ethics of Animal Experiments of Barrow Neurological Institute and Tianjin Neurological Institute. All surgeries were performed under isoflurane anesthesia.
4
Genetically Modified Mouse Strains
5
LPS-Induced Inflammatory Response in Mice
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Eight to nine week‐old female C57Bl/6 (B6) mice (Taconic) were used. One group of mice (N = 10) was injected intraperitoneally with 20 µg/g LPS (Escherichia coli) (Sigma, Serotype 055:B5, L‐2880) dissolved in sterile saline. Another group of animals (N = 8) was sham‐injected with sterile saline. The animals were fed with standard laboratory chow, had free access to water and food, and were killed by cervical dislocation 6 hr after injection. The mice were housed in a high quality barrier with high hygienic standards and housed and experimentally manipulated in accordance with current national regulations issued by The Danish Council on Animal Care.
6
C57BL/6 Skin Transplant Protocol
7
Helicobacter pylori Infection in Mice
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Mice were maintained in a facility accredited by the Association for the Assessment and Accreditation of Laboratory Animal Care. All animal care and experimental procedures were approved by the Massachusetts Institute of Technology Committee on Animal Care and Use in accordance with the Guide for the Care and Use of Laboratory Animals. Fifteen 4-week-old female C57BL/6 (B6) mice were purchased from Taconic Biosciences (Tac) (Germantown, NY) and the Jackson Laboratory (Jax) (Bar Harbor, ME), respectively. The mice were free of known murine viruses, Helicobacter spp. and parasites, and were maintained in static microisolator cages and fed a diet (ProlabRMH3000) from PMI Nutrition International (Richmond, IN) . After being in quarantine for a week, 10 mice from each vendor were inoculated with H. pylori PMSS1 (containing a functional CagA), whereas 5 control mice of each vendor, which were housed in a separate cubicle, were sham-dosed with vehicle. Mice received 0.2 ml of fresh inocula (~2 × 108 organisms) by gastric gavage every other day for a total of three inoculations.
8
Generation and Characterization of Transgenic Mice
9
Eosinophil-specific Fluorescent Reporter Mice
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C57BL/6 (B6) mice were purchased from Taconic Farms (Hudson, NY). B6.SJL (CD45.1/1), B6.SJL/ C57BL/6 (CD45.1/2) mice were obtained through a supply contract between the National Institute of Allergy and Infectious Diseases (NIAID) and Taconic Farms. Epx-Cre mice (Doyle et al., 2013) were initially obtained from Elizabeth Jacobsen (Mayo Clinic, Phoenix, AZ) and subsequently crossed to ROSA26-LSL-tdTomato mice to generate eosinophil-specific fluorescent EPX-reporter animals and generously gifted by David Sacks (NIAID) (Lee et al., 2020b) . Gpr183 -/-mice (Baptista et al., 2019; Liu et al., 2011)
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