Ion trap lc ms ms spectrometer

Gels were rinsed with HPLC-grade water. Excised bands were destained in 100 µL of 100 mM ammonium bicarbonate/acetonitrile solution for 30 minutes at room temperature and then washed with 500 µL of neat acetonitrile. Gel pieces were then covered with 10 ng/µL porcine trypsin solution in 10 mM ammonium bicarbonate/10% (v/v) acetonitrile and incubated for 2 h on ice followed by overnight incubation at 37 °C. After digestion, samples were centrifuged and supernatant aliquots were withdrawn and stored at −20 °C until LC MS/MS analysis. The analyses were performed on an Ion Trap LC/MS/MS spectrometer (Agilent Technologies, Santa Clara, CA USA). The resulting peptide mass fingerprints and LC MS/MS fragmentation spectra were identified using the MASCOT (http://www.matrixscience.com) and BLAST engines [53 (link)] searching M. catarrhalis protein databases.

The analyses were performed on an Ion Trap LC/MS/MS spectrometer (Agilent Technologies, Santa Clara, CA USA). The resulting peptide mass fingerprints and LC MS/MS fragmentation spectra were identified using the MASCOT (http://www.matrixscience.com) and BLAST engines^{56 (link)} searching M. catarrhalis protein databases.