RPMI 1640 was purchased from Lonza (Basel, Switzerland), and antibiotic solution (100 U/ml penicillin and 100 μg/ml streptomycin) was from Invitrogen Inc. (Carlsbad, CA, US). Monoclonal anti-human IκB-α, phospho-p38 mitogen-activated protein kinase (MAPK), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibodies were from Cell Signalling (Danvers, MA, US). Horseradish peroxidase-conjugated secondary antibodies were from Vector (Peterborough, UK). Anti-CCR2 mAb, anti-CD163 mAb, anti-IL-1β mAb, brefeldin, Fix, and Perm buffer solutions were from eBioscience/Affymetrix (Santa Clara, CA, US); anti-CD80 mAb and anti-CD206 mAb were from BD Biosciences Pharmigen (San Diego, CA, US). The cOmplete™ inhibitor cocktail was from Roche Diagnostics (Mannheim, Germany). Fetal bovine serum (FBS), Ficoll-Paque (density 1.077 ± 0.001), Percoll, dexamethasone, curcumin, CLI-095, skim milk powder as well as other analytic grade chemical agents were from Sigma-Aldrich. Ultrapure LPS (LPS-EB), zymosan (ZYMO), Pam3CSK4, and Poly(I:C) were from InvivoGen (San Diego, CA, USA). IL-4, IL-13, and colony stimulating factor-1 (CSF-1) were from ImmunoTools (Friesoythe, Germany). The curcumin analogues GG6 and GG9 were synthesized as described elsewhere [13 (link)].
Glyceraldehyde 3 phosphate dehydrogenase gapdh antibody
Manufactured by Cell Signaling Technology
Sourced in United States
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody is a primary antibody that recognizes the GAPDH protein. GAPDH is an enzyme involved in the glycolytic pathway, catalyzing the conversion of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate.
Automatically generated - may contain errors
Lab products found in correlation
α sma antibody, by Merck Group (2 mentions)
Alexa fluor 488 goat anti mouse immunoglobulin g igg, by Thermo Fisher Scientific (2 mentions)
Trizol reagent, by Thermo Fisher Scientific (2 mentions)
Antibiotic solution, by Thermo Fisher Scientific (1 mentions)
Skim milk powder, by Merck Group (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Complete inhibitor cocktail, by Roche (1 mentions)
Cli095, by Merck Group (1 mentions)
Horseradish peroxidase conjugated secondary antibody, by Vector Laboratories (1 mentions)
Ficoll paque, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Poly 1 c, by InvivoGen (1 mentions)
Il 13, by Immunotools (1 mentions)
Curcumin, by Merck Group (1 mentions)
Dexamethasone (dex), by Merck Group (1 mentions)
Rpmi 1640, by Lonza (1 mentions)
Interleukin 4 (il 4), by Immunotools (1 mentions)
Enhanced chemiluminescence ecl solution, by Beyotime (1 mentions)
Radio immunoprecipitation assay ripa lysis buffer, by Beyotime (1 mentions)
10 protocols using glyceraldehyde 3 phosphate dehydrogenase gapdh antibody
1
Macrophage Polarization Assay
2
A. villosum Fruit Bioassays
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The dried fruit of A. villosum was purchased from Yangchun City Spring Gold Agriculture Investment Co., Ltd. (Yangchun, China) and identified by Dr. Zhengming Qian (Dongguan HEC Cordyceps R&D Co., Ltd., Dongguan, China). The voucher specimen (ZMU‐20) was deposited in the research and development center. Assay kits for aspartate aminotransferase (AST), alanine aminotransferase (ALT), total bilirubin (TBIL), low‐density lipoprotein cholesterol (LDL‐C), high‐density lipoprotein cholesterol (HDL‐C), glutathione (GSH), glutathione peroxidase (GSH‐Px), total cholesterol (TC), and triglycerides (TG) were obtained from Nanjing Jiancheng Bioengineering Institute (Jiangsu, China). ELISA kits for rat tumor necrosis factor‐α (TNF‐α) and interleukin 6 (IL‐6) were purchased from Nanjing Senberga Biotechnology Co., Ltd. (Jiangsu, China). The bicinchoninic acid (BCA) protein assay kit, radioimmunoprecipitation assay (RIPA) lysis buffer, and enhanced chemiluminescence (ECL) solution were obtained from Beyotime (Shanghai, China). Primary antibody against estrogen receptor 1 (ESR1) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA), while anti‐nuclear receptor subfamily 3 group C member 1 (NR3C1) and glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) antibodies were obtained from Cell Signaling Technology (Beverly, MA, USA). All reagents used in this study were of analytical grade.
3
Signaling Pathways in Adipocyte Metabolism
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Antibodies against peroxisome proliferator-activated receptor gamma co-activator 1 alpha (PGC1A) and the p38 mitogen-activated protein kinase (MAPK) inhibitor SB 202190 were purchased from Calbiochem. Phosphospecific p38 MAPK, total p38 MAPK and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibodies were obtained from Cell Signalling. TOMM20 antibodies were purchased from Abnova (Taipei, Taiwan). ATP synthase beta antibodies and the specific D1-like agonist SKF 38393 were from Abcam. Specific UCP1 and D1-like receptor antibodies were purchased from Chemicon International. Specific D2-like receptor antibodies and the specific D2-like antagonist raclopride were from Santa Cruz Biotechnology. The specific D1-like antagonist SCH 23390 and D2-like agonist bromocriptine were purchased from Tocris Bioscience (Bristol, UK). Secondary antibodies were from Life Technologies. Fatty acid-free bovine serum albumin (BSA) was from Serva (Heidelberg, Germany). All other materials were obtained from Sigma-Aldrich.
4
LPS-Induced Inflammasome Activation
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LPS (E coli 0111:B4) and adenosine triphosphate (ATP) were obtained from Sigma-Aldrich (St. Louis, MO). Rabbit polyclonal caspase-1 P10 (M-20) antibody was sourced from Santa Cruz, CA. Rabbit polyclonal TLR4, IRF-1, IL-1β, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody were all from Cell Signaling Technology (Boston, MA). Rabbit polyclonal Histone H3 antibody and Rabbit polyclonal ASC antibody was obtained from ImmunoWay Biotechnology Co (Newark, DE). Alexa555-conjugated secondary antibody was obtained from Molecular Probes Inc (Eugene, OR).
5
Tumorsphere Culture of NSCLC Cell Lines
6
Cytochrome C Protein Detection
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Cultured ASMCs were frozen and lysed in lysis buffer. Total proteins were separated by 8% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto a nitrocellulose membrane. Block membrane [in 30 mL phosphate buffered saline (PBS) + 5% non-fat dry milk + 0.1% Tween 20 in a small dish on a shaker] was then incubated with cytochrome C antibody (1:1,000; Cell Signaling Technology, USA). Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibody (1:1,000; Cell Signaling Technology, USA) was used for control.
7
Antibody Characterization for Cell Signaling Assays
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Phospho‐Akt (Ser473 and Thr308) antibody, phospho‐GSK‐3α (Ser21)/GSK‐3β (Ser9) antibody, phospho‐p38 (Thr180/Tyr182) antibody, Akt antibody, GSK‐3α/β antibody, p38 antibody, and glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) antibody were purchased from Cell Signaling (Danvers, MA, USA). α‐SMA antibody was purchased from Sigma‐Aldrich (St. Louis, MO, USA). Alexa Fluor 488 goat anti‐mouse immunoglobulin G (IgG) and 4′,6‐diamidino‐2‐phenylindole (DAPI) were purchased from Invitrogen (Carlsbad, CA, USA). ML221 was purchased from Sigma‐Aldrich. LY294002 was purchased from Cell Signaling.
8
Cell Counting and Protein Expression Analysis
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The cell counting kit-8 (CCK-8) assay kit was obtained from Signal way& Antibody (SAB, MA, USA). Trizol reagent and reverse transcription kits were purchased from Thermo Fisher (MA, USA). Crystal violet was obtained from Solarbio (Shanghai, China). FAM46B and vascular endothelial growth factor (VEGF) antibodies were purchased from Proteintech (IL, USA). β-catenin and matrix metalloproteinase 7 (MMP7) antibodies were obtained from Abcam Biotech (Cambridge, MA, USA). Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibody was purchased from Cell Signaling Technology (MA, USA).
9
Molecular Signaling Pathway Analysis
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Galectin‐3 antibody, SAPK/JNK antibody, phospho‐SAPK/JNK (Thr183/Tyr185) antibody, Phospho‐PI3 Kinase Class III (Ser249) antibody, PI3 Kinase Class III antibody, SQSTM1/p62 antibody, LC3B antibody, and glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) antibody were purchased from Cell Signaling (Danvers, MA, USA). Collagen type 1A1 (COL1A1) antibody and interleukin (IL)‐1R‐associated kinase 1 (IRAK‐1) were purchased from Santa Cruz (Dallas, TX, USA). The α‐SMA antibody was purchased from Sigma‐Aldrich (St. Louis, MO, USA). Alexa Fluor 488 goat anti‐mouse immunoglobulin G (IgG) and Alexa Fluor 594 goat anti‐rabbit immunoglobulin G were purchased from Invitrogen (Carlsbad, CA, USA). DOX hydrochloride and SP600125 were purchased from Sigma‐Aldrich. Chloroquine was purchased from Thermo Fisher Scientific (Waltham, MA, USA). Pioglitazone hydrochloride was purchased from Fujifilm (Tokyo, Japan). ODN2088 and GW1929 were purchased from AdipoGen Life Sciences (San Diego, CA, USA).
10
Melamine-Induced Hepatic Injury Study
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Melamine [2,4,6-triamino-s-triazine, CAS No. 108-78-1] was purchased from Tokyo Chemical Industry Co., Ltd. (Shanghai, China). Choline chloride was purchased from Jinan Asia Pharmaceutical Co., Ltd. (Jinan, China). Ethylene Glycol was purchased from Beijing Tongguang Fine Chemical Co., Ltd. (Beijing, China). C–X–C motif chemokine ligand 14 (CXCL14) antibody, F4/80 antibody and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody were purchased from Cell Signaling Technology (Danvers, MA, USA). All other chemicals were of analytical grade and used as received.
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