Anti integrin α5

Manufactured by Abways

Anti-integrin α5 is a laboratory reagent that binds to the α5 subunit of the integrin receptor. Integrins are cell adhesion molecules that play a role in cell-cell and cell-extracellular matrix interactions. This product can be used to study the functions and signaling pathways associated with the α5 integrin subunit.

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Lab products found in correlation

Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions) Anti sqstm1 p62, by Cell Signaling Technology (2 mentions) Pierce classic magnetic ip co ip kit, by Thermo Fisher Scientific (2 mentions) Anti glyceraldehyde 3 phosphate dehydrogenase gapdh, by Cell Signaling Technology (2 mentions) Anti atg5, by Abways (2 mentions) Anti lc3b, by Cell Signaling Technology (2 mentions) Anti ulk1, by Cell Signaling Technology (1 mentions) Ab32419, by Abcam (1 mentions) Ab207612, by Abcam (1 mentions) Anti phospho ulk1 ser757, by Cell Signaling Technology (1 mentions) Ass1009, by Abcepta (1 mentions) Af3308, by Affinity Biosciences (1 mentions) Anti tlr4, by Affinity Biosciences (1 mentions) Anti mtor, by Affinity Biosciences (1 mentions) Anti rab7, by Cell Signaling Technology (1 mentions) Hiperfect, by Qiagen (1 mentions)

2 protocols using anti integrin α5

Western Blot Analysis of Autophagy Markers

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For Western blot analysis, the following antibodies were used: anti-LC3B (2775; Cell Signaling Technology), anti-SQSTM1/P62 (5114; Cell Signaling Technology), anti-ATG5 (CY5766; Abways), anti-fibronectin (ab32419; Abcam), anti-integrin α5 (CY5979; Abways), anti-integrin β1 (CY5469; Abways), anti-phospho-mTOR (Ser2448) (AF3308; Affinity Biosciences), anti-mTOR (AF6308; Affinity Biosciences), and anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (5174; Cell Signaling Technology). Horseradish peroxidase-labeled goat anti-rabbit (ASS1009; Abgent) secondary antibodies were used. The transfection reagents were Lipofectamine 2000 (11688-019; Invitrogen) and Lipo6000™(Beyotime). The immunoprecipitation (IP) reagent was included in the Pierce classic magnetic IP/co-IP kit (88804; Thermo Scientific).

Meng M., Wang J., Li H., Wang J., Wang X., Li M., Gao X., Li W., Ma C, & Wei L. (2024). Eliminating the invading extracellular and intracellular FnBp+ bacteria from respiratory epithelial cells by autophagy mediated through FnBp-Fn-Integrin α5β1 axis. Frontiers in Cellular and Infection Microbiology, 13, 1324727.

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Western Blot Antibody Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

For Western blot analysis, the following antibodies were used: anti-LC3B (2775; Cell Signaling Technology), anti-SQSTM1/P62 (5114; Cell Signaling Technology), anti-ATG7 (DF6130; Affinity Biosciences), anti-ATG5 (CY5766; Abways), anti-fibronectin (ab32419; Abcam), anti-integrin α5 (CY5979; Abways), anti-integrin β1 (CY5469; Abways), anti-phospho-Beclin-1 (Ser15) (84966; Cell Signaling Technology), anti-BECN1 (ab207612; Abcam), anti-TLR2 (AF7002; Affinity Biosciences), anti-TLR4 (AF7017; Affinity Biosciences), anti-CST (AB0055; Abways), anti-Rab7 (9367; Cell Signaling Technology), anti-phospho-MTOR (Ser2448) (AF3308; Affinity Biosciences), anti-MTOR (AF6308; Affinity Biosciences), anti-phospho-ULK1 (Ser757) (6888; Cell Signaling Technology), anti-ULK1 (8054; Cell Signaling Technology), and anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (5174; Cell Signaling Technology). Horseradish peroxidase-labeled goat anti-rabbit (ASS1009; Abgent) secondary antibodies were used. The transfection reagents were Lipofectamine 2000 (11688-019; Invitrogen) and HiPerFect (Y5-301705; Qiagen). The immunoprecipitation (IP) reagent was included in the Pierce classic magnetic IP/co-IP kit (88804; Thermo Scientific).

Wang J., Meng M., Li M., Guan X., Liu J., Gao X., Sun Q., Li J., Ma C, & Wei L. (2020). Integrin α5β1, as a Receptor of Fibronectin, Binds the FbaA Protein of Group A Streptococcus To Initiate Autophagy during Infection. mBio, 11(3), e00771-20.

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