The reagents used in this study were purchased from the following suppliers: Prestained protein markers and Bio-Rad assay reagents and standards (Bio-Rad Laboratories, Hercules, CA); Protran BA83 Nitrocellulose membrane (Whatman Inc, Sanford, ME); Pierce ECL (Pierce Biotechnology, Rockford, IL); protease inhibitor cocktail tablets complete EDTA-free and PhosStop (Roche Applied Biosciences, Indianapolis, IN); antibiotic-antimycotic, and trypsin–EDTA (Invitrogen Life Technologies Inc, Carlsbad, CA); Blue X-Ray film (Phenix Research Products, Hayward, CA); fetal bovine serum (HyClone, Logan, UT); and tissue culture dishes and plates (Corning Inc, Corning, NY). Antagonists/inhibitors for EP2 (AH6809), EP4 (AH23848) were purchased from Sigma (Sigma-Aldrich, St-Louis, MO). All other antibodies used in this study were purchased from Cell Signaling Technology (Danvers, MA), Chemicon International (Billerica, MA), or Santa Cruz Biotechnology (Santa Cruz, CA) except β-actin monoclonal antibody (Sigma-Aldrich), goat anti-rabbit or anti-mouse IgG conjugated with horseradish peroxidase (Kirkegaard & Perry Laboratories, Gaithersburg, MA). The chemicals used were molecular biological grade from Fisher Scientific (Pittsburgh, PA) or Sigma-Aldrich (St. Louis, MO).
Assay reagents and standards
Manufactured by Bio-Rad
Sourced in United States
Bio-Rad assay reagents and standards are laboratory products designed to facilitate various analytical and experimental procedures. These products include a range of reagents, buffers, and reference standards used in common laboratory techniques such as protein quantification, enzyme activity assays, and immunoassays. The core function of these products is to provide consistent and reliable components to support accurate and reproducible data generation in research and diagnostic applications.
Automatically generated - may contain errors
Lab products found in correlation
Whatman, by Cytiva (2 mentions)
Protran, by Cytiva (2 mentions)
β actin monoclonal antibody, by Merck Group (1 mentions)
Phosstop, by Roche (1 mentions)
Tissue culture dishes and plates, by Corning (1 mentions)
Prestained protein markers, by Bio-Rad (1 mentions)
Protease inhibitor, by Roche (1 mentions)
Vectastain elite abc kit, by Vector Laboratories (1 mentions)
2 protocols using assay reagents and standards
1
Comprehensive List of Reagents Used
2
Western Blot Reagents and Antibodies
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Prestained protein markers, Bio-Rad assay reagents and standards (Bio-RAD Laboratories); protran BA83 Nitrocellulose membrane (Whatman, Inc., Sanford, ME, USA); pierce ECL (Pierce, Rockford, IL, USA); protease inhibitor (Roche Applied Biosciences); Vectastain Elite ABC kit (Vector Laboratories, Inc., Burlingame, CA, USA); Progesterone RIA kits (Diagnostic Systems Laboratories, Webster, TX, USA); Blue X-ray film (Phoenix Research Products, Hayward, CA, USA) were purchased. Details of antibodies used in this study are given in Table 1. The other chemicals used were molecular biological grade from Fisher (Pittsburgh, PA, USA) or Sigma-Aldrich.
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