Nupage novex system
The NuPage Novex system is a lab equipment product designed for electrophoresis separation and analysis of proteins. It features pre-cast polyacrylamide gels, buffers, and accessories to facilitate consistent and reliable protein sample preparation and separation.
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23 protocols using nupage novex system
Western Blotting of Adenosine Receptors
Analysis of Selenoprotein P by Mass Spectrometry
Protein Extraction and Immunoblotting Procedure
SDS-PAGE and immunoblotting was performed with the Novex NuPAGE system (Life Technologies) according to the manufacturer's recommendations. The protocol is described in detail elsewhere [20] (link). The following primary antibodies were used: anti TP53 central parts (Pab240, diluted 1∶200, Santa Cruz Biotechnology), anti TP53 N-terminal (DO-1, diluted 1∶200, Calbiochem Merck), anti TP53 C-terminal (Pab421, diluted 1∶200, Calbiochem Merck), anti DDIT3 (15204-1-AP, diluted 1∶266, Proteintech) and anti GAPDH (mAbcam 9484, diluted 1∶200, Abcam)
Fluorometric Assessment of Cathepsin Enzymes
Western Blot Protein Analysis Protocol
Mitochondrial Proteome Analysis by Western Blot
Protein Expression Analysis in Cartilage Cells
Protein Purification and Quantification
Western Blot Analysis of Pericentrin
Characterization of Anti-BLG Antibodies
Bovine BLG (120 µg/480 µl non-reducing sample buffer) was loaded and electrophoresed in a one well pre-casted 4–12% BisTris gel/MOPS running buffer NuPage Novex system (Invitrogen). Then, bovine BLG was electro-blotted onto a polyvinylidene fluoride transfer membrane (Millipore). After blocking with PBS/0.05% Tween 20/1% BSA fraction V (Roche) for 1 hour at RT, this membrane was incubated with 1 µg/ml purified chimeric human IgE anti-BLG antibody clones 5D6.1, 7D5.1, 8C3.3, 8F7.1, 11B6.2 or 13A5.2 for 1 hour at RT. After washing in PBS/0.05% Tween 20, binding of these chimeric human IgE anti-BLG antibodies was determined with 1∶4,000 diluted HRP-conjugated goat anti-human IgE-specific antibodies (Sigma) for 1 hour at RT, followed by a ready-to-use solution of TMB substrate (Sigma) for colorimetric detection.
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