Interleukin il 1β

We performed evaluations on soluble factors from supernatants of uncultured mSVF and eSVF through the commercially enzyme-linked immunosorbent tests (ELISA). To this end, mSVF and eSVF were centrifuged at 600 g for 10 min, and their supernatants were collected to test the released cytokines. Moreover, we plated and cultured mSVF and eSVF to test whether the in vitro culture can modify their cytokine profile compared to the native uncultured adipose products. After 80% confluence, culture supernatants (S/N) from both mSVF (mSVF S/N) and eSVF (eSVF S/N) were recovered and kept in serum-free medium for 48 h before their collection. We performed ELISA assessments on the following molecules: vascular endothelial growth factor (VEGF) (Cloud-Clone Corp, Texas, US), Platelet-derived growth factor (PDGF)-bb (LifeSpan Biosciences, Washington, US), insulin growth factor (IGF)-1 (Cloud-Clone Corp), hepatocyte growth factor (HGF) (Cloud-Clone Corp), interleukin (IL)-10 (Cloud-Clone Corp), interleukin (IL)-1β (Cloud-Clone Corp), IL-6 (Cloud-Clone Corp), IL-21 (MyBioSource, California, US), IL-23 (MyBioSource), tumour necrosis factor (TNF)-α (Cusabio, Houston, Texas, US). We used the manufacturer’s instructions through spectrophotometer measurement at a wavelength of 510–570 nm.

The levels of cytokines in the prefrontal cortex were determined using ready-made for mice ELISA Kits (Interleukin IL-1β, IL-6 and TNF-α, Cloud-Clone Corp., Houston, TX, USA). We performed the determinations in accordance with the manufacturer’s instructions (S2).