Imperatorin

RPMI-1640, Modified Eagle Medium (MEM), non-essential amino acid solution, and penicillin-streptomycin were acquired from Hyclone (Logan, Utah, USA). Imperatorin was purchased from ChromaDex (Irvine, CA, USA). Mouse anti-dinitrophenyl (DNP) IgE and DNP-human serum albumin (HAS) were obtained from Sigma-Aldrich (St. Louis, MO, USA). The recombinant mouse SCF was purchased from STEMCELL Technologies Inc (Vancouver, BC, Canada). The primary antibodies used in the experiments were as follows: rabbit polyclonal antibodies specific for phospho-ERK1/2, ERK1/2, phosphop38, p38, phospho-JNK, JNK, phospho-PLCγ1, phospho-Akt, Akt, phospho-IκBα, IκBα, phospho-IKKα/β, and β-actin from Cell Signaling Technology, Inc. (Beverly, MA, USA); rabbit polyclonal antibodies for 5-LO, and phospho-cPLA₂ from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Specific inhibitors for NF-κB (pyrrolidine dithiocarbamate, PDTC) and MAPKs (SB203580, PD98059, and SP600125) were obtained from Sigma. The horseradish peroxidase-conjugated goat anti-rabbit secondary antibody was from Cell Signaling. 1×RIPA buffer, NE-PER Nuclear Protein Extraction kit, phosphatase/protease inhibitor cocktail, and enhanced chemiluminescence (ECL) detection reagent were from Pierce (Rockford, IL, USA). The enzyme immunoassay (EIA) kits for LTC₄ and PGD₂, and COX-2 antibody were purchased from Cayman Chemicals (Ann Arbor, MI, USA).

Imperatorin was purchased from ChromaDex (Irvine, CA, USA). Dulbecco's modified eagle's medium (DMEM) and fetal bovine serum (FBS) were obtained from Invitrogen (Carlsbad, CA, USA). Insulin, transferrin, γ-amino butyric acid, poly-L-lysine, cytosine arabinoside, MK801, diltiazem, and nifedipine were from Sigma-Aldrich (St. Louis, MO, USA). The ERK and phospho-ERK antibodies were from Cell Signaling Technology (Beverly, MA) and GAPDH antibody was from Santa Cruz Biotechnology (Santa Cruz, CA, USA).