Plv3 vector

Manufactured by GenePharma

Sourced in China

The PLV3 vector is a laboratory tool used for gene expression and cloning. It serves as a plasmid-based vector that can be used to introduce DNA sequences into target cells or organisms. The core function of the PLV3 vector is to facilitate the incorporation and expression of genetic material within a controlled experimental environment.

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Lab products found in correlation

Recombinant human il 1ra, by Thermo Fisher Scientific (1 mentions) β actin, by Merck Group (1 mentions) Bay11 7082, by Merck Group (1 mentions) Recombinant human il 1β, by Thermo Fisher Scientific (1 mentions) Il 1r1, by Santa Cruz Biotechnology (1 mentions) Bcl 2, by Santa Cruz Biotechnology (1 mentions)

2 protocols using plv3 vector

Molecular Mechanisms of Inflammation

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Constructs: HNF4α siRNA (Sigma-aldrich), HNF4α shRNA constructed in pLV3 vector (GenePharma), Bay 11-7082 (Sigma-aldrichInc), MNU(Sigma-aldrich), Recombinant Human IL-1β(Peprotech), Recombinant Human IL-1RA(Peprotech);
Antibodies: HNF4α(CST), p65(Abcam), IL-1R1(Santa Cruz), p-p65(Cell Signaling), cagA(Abcam), ki67(Abcam)CCND1(Cell Signaling), Bcl-2(Santa Cruz), b-actin(Sigma aldrich).

Ma L., Zeng J., Guo Q., Liang X., Shen L., Li S., Sun Y., Li W., Liu S., Yu H., Chen C, & Jia J. (2016). Mutual amplification of HNF4α and IL-1R1 composes an inflammatory circuit in Helicobacter pylori associated gastric carcinogenesis. Oncotarget, 7(10), 11349-11363.

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Generation of RBP2 knockdown cells

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BGC-823 cells with stable knockdown were generated using short hairpin RNAs (shRNA) directed against human RBP2 gene constructed in pLV3 vector got from GenePharma (Shanghai, China). Control cells were created by using a plasmid carrying nontargeting control sequence.

Liang X., Zeng J., Wang L., Shen L., Li S., Ma L., Ci X., Yu J., Jia M., Sun Y., Liu Z., Liu S., Li W., Yu H., Chen C, & Jia J. (2014). Histone demethylase RBP2 induced by Helicobactor Pylori CagA participates in the malignant transformation of gastric epithelial cells. Oncotarget, 5(14), 5798-5807.

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