Leica hc pl apo

Manufactured by Leica camera

Sourced in Germany

The Leica HC PL APO is a high-end microscope objective lens designed for advanced laboratory applications. It provides excellent optical performance with a high numerical aperture, ensuring crisp and detailed image quality. The lens is optimized for polarized light microscopy, offering true color reproduction and minimal chromatic aberration.

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Lab products found in correlation

Cellmask red, by Thermo Fisher Scientific (1 mentions) Tcs sp8 confocal microscope, by Leica camera (1 mentions)

2 protocols using leica hc pl apo

Visualizing Tetherin on Primary Monocytes

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Primary monocytes were isolated as specified above, fixed using 2% paraformaldehyde, and stained for tetherin as specified for scanning electron microscopy, followed by secondary goat anti‐rabbit H&L Alexa Fluor 488 (Molecular Probes). The cellular membrane was labeled using Cell Mask Red (Invitrogen). Images were acquired using a Leica (Wetzlar, Germany) TCS SP8 confocal microscope with Leica HC PL APO CS2 ×63 magnification with 1.40 numerical aperture at room temperature. The acquisition software used was Leica LAS X (BETA) 3.5.0.18154 with Leica detectors HyD 505 to 588 nm in standard mode and HyD 654 to 731 nm in standard mode. Images were processed with Leica LIGHTNING Image Information Extraction.

Weber E.A., Singh M.V., Singh V.B., Jackson J.W., Ture S.K., Suwunnakorn S., Morrell C.N, & Maggirwar S.B. (2020). Novel Mechanism of Microvesicle Regulation by the Antiviral Protein Tetherin During HIV Infection. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 9(17), e015998.

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Intracellular Localization of Gag-PLK Complexes

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The analysis of the intracellular distribution of C-terminal mCherry-tagged Gag constructs and N-terminal eGFP-tagged PLK constructs using confocal microscopy was done as described previously [37 (link)]. Briefly, 293T cells were co-transfected with 1.7 μg of Gag-mCherry-encoding and 2 μg of eGFP-PLK encoding construct, using PEI-transfection method in 100 mm dishes as described above. One day post-transfection, cells were replated at a concentration of 3 x 10⁵ cells per well on poly-L-lysine coated cover slips in 12-well plates. At 48 h post-transfection the cells were washed with cold PBS, fixed with 3% paraformaldehyde, and the cell nuclei were stained with DAPI for 5 min. Finally the cells were covered with Mowiol. Confocal laser scanning images were obtained on a Leica SP5, using a Leica HC PL APO 40x 1.25 oil immersion objective. Fluorescence images were evaluated using ImageJ software. For quantitative analysis of co-localization at least 100 cells showing co-expression of individual Gag-PLK protein combinations were evaluated.

Zurnic I., Hütter S., Rzeha U., Stanke N., Reh J., Müllers E., Hamann M.V., Kern T., Gerresheim G.K., Lindel F., Serrao E., Lesbats P., Engelman A.N., Cherepanov P, & Lindemann D. (2016). Interactions of Prototype Foamy Virus Capsids with Host Cell Polo-Like Kinases Are Important for Efficient Viral DNA Integration. PLoS Pathogens, 12(8), e1005860.

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