Streptavidin peroxidase detection system
The Streptavidin peroxidase detection system is a lab equipment product used to detect and quantify the presence of biotinylated molecules in a sample. It utilizes the high-affinity binding between streptavidin and biotin to amplify the signal, providing a sensitive and reliable method for analysis.
Lab products found in correlation
7 protocols using streptavidin peroxidase detection system
Immunohistochemical and Immunofluorescent Analyses
Immune Cell Profiling in Kidney Tissue
Renal Tissue Analysis: PAS Staining and Immunohistochemistry
Immunohistochemical Analysis of Cx43 and Podocin
Immunohistochemical Evaluation of Kidney Injury Markers
Immunohistochemistry and Immunofluorescence Staining of IgA in Intestine
The kidney and small intestine tissue for IF staining was cut into frozen sections and fixed with acetone for 1 min. After fixation, they were blocked with 2% bovine serum albumin diluted by PBS at room temperature for 1 h. They were washed with PBS three times and incubated with FITC-labelled goat anti-mouse IgA (Abcam, ab97234) at 37°C for 40min. After washing with PBS three times, they were mounted with anti-quenching tablets and observed under a confocal microscope. Cell nuclei were stained with DAPI. Images were quantified by counting the number of positive nuclei and divided by the total number of nuclei.
Immunohistochemical Analysis of NLRP3 Inflammasome
For analysis and localizing the expression of the NLRP3 and cathepsin B, immunofluorescence staining of tissue sections or formaldehyde-fixed cells was performed using anti-NLRP3, anti-cathepsin B antibodies (Abcam, USA), respectively in a humidified chamber overnight at 4 °C, followed by incubation with an Alexa fluorescein-labeled secondary antibodies (Invitrogen, USA) for 1 h. Cell nuclei were stained with DAPI. Immunostained samples were visualized under a confocal microscope. Immunofluorescence for p65 (Cell Signaling Technology, USA) was similarly performed. Quantification of intensity of p65 in nuclear was performed by measuring area, integrated density, and mean gray value using Image-Pro Plus.
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