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Cdh1 610181

Manufactured by BD
Sourced in United States

CDH1 (610181) is a DNA-binding protein that plays a crucial role in cell-cell adhesion and the maintenance of tissue architecture. It serves as a component of the adherens junction complex, which is important for the organization and function of epithelial cells. The CDH1 product is designed for research purposes and its core function is to facilitate the study of cell-cell adhesion and related cellular processes.

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2 protocols using cdh1 610181

1

Immunohistochemistry of Fetal Lung Tissue

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Immunohistochemistry was performed on formalin-fixed, paraffin-embedded human fetal lungs (T21 and non-T21) as previously described [27 (link)]. In brief, paraffin-embedded lungs were sectioned at 5 μm, rehydrated, and then boiled in a sodium citrate antigen retrieval solution for 12 min. A 15-min step in 3% H2O2 was performed to quench endogenous peroxidases to decrease background. Sections were incubated with the following primary antibodies overnight at 4 °C: CD31 (RB-10333-P0, 1:200; Neomarkers, Fremont, CA, USA), CDH1 (610181, 1:200; BD Biosciences, San José, CA, USA), Ki67 (RM-9106-S1, 1:200; Thermo Fisher Scientific, Fremont, CA, USA), LYVE1 (ab14917, 1:100; Abcam, Cambridge, UK), SOX2 (sc-17320, 1:100; Santa Cruz, Dallas, TX, USA), and SOX9 (AB5535, 1:500; Millipore, Burlington, MA, USA). Sections were then stained with appropriate fluorochrome-conjugated secondary antibodies in conjunction with Cy3-ACTA2 conjugated antibody (C6198, 1:200; Sigma-Aldrich, St Louis, MO, USA) when necessary. Slides were counterstained with DAPI (DE571; Life Technologies, Grand Island, NY, USA) and mounted using ProLong Diamond Antifade Mountant (Life Technologies).
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2

Immunoblotting of Cellular Signaling Proteins

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Cell and epidermal lysates prepared as previously described (Doma et al., 2013 (link)) were immunoblotted using the following primary antibodies (1:1000): TUBA (T9206,) from Sigma; pERK1/2 (9101), ERK1/2 (9102), JNK1/2 (9258), pJNK 1/2 (9251), pJUN (9164), pMAPKAPK2 (3041), pp38 (9211), p38 (9212) and ICAM1 (4915) from Cell Signaling Technology; ACTB (sc-1616), 14-3-3 (sc-1657), BRAF (sc-5284), RAF1 (sc-133) and MLK3 (sc-166639 and sc-536) from Santa Cruz Biotechnology (Dallas, TX); CDH1 (610181) from BD Biosciences; OCLN (ab31721), pRSK (ab32413), TSLP (ab188766) and DUSP10 (ab140123) from Abcam; GAPDH (ABS16) and DUSP1 (07–535) from Millipore; ICAM1 (AF796, R&D Systems) and CLDN1 (374900, Invitrogen, Karlsbad, CA).
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