Labsolutions workstation

Two Nexera LC-20AD_XR pumps, a SPD-20A UV/VIS detector, a CBM-20A communication bus module, an LCMS-8040 triple quadruple mass spectrometer, and a Lab Solutions work station (Shimadzu Corporation, Kyoto, Japan) were employed in the UPLC-ESI-MS/MS system. A UPLC column (Shim-pack XR-ODS II, 75 × 2.0 mm I.D., 3 μm, Shimadzu Corporation, Kyoto, Japan) was maintained at 37 °C in a CTO-20AC column oven. The following UPLC-ESI-MS/MS system conditions were used: a mobile phase of acetonitrile-water (0.5% acetic acid) (40:60, v/v) with a 0.4 mL min⁻¹ flow rate and UV detection. The MS/MS conditions were: nebulizer gas (N₂, purity > 99.999%), flow rate of 3.0 L min⁻¹; drying gas (N₂, purity > 99.999%), flow rate of 15.0 L min⁻¹; interface, ESI source; desolvation line (DL) temperature, 250 °C; heat block temperature, 400 °C; interface voltage, 4.5 kV, interface current, 4.7 μA; detector voltage, 1.72 kV; CID gas (Ar, purity > 99.999%), pressure, 230 kPa; multiple reaction monitoring (MRM) mode. The detailed MRM method of histamine serotonin and PGE2 was shown in Supplementary Table 5. The detailed multiple reaction monitoring method of β-hexosaminidase and TNF-α after trypsin digestion was shown in Supplementary Table 6.

The following instruments were used in our study: a Shimadzu LC-20AT pump with an SPD-M20A photodiode array detector and LabSolutions workstation (Shimadzu Corporation, Kyoto, Japan), a Q-Trap 3200 (AB Sciex, Foster City, CA, USA), and a Varian INOVA 600 MHz NMR spectrometer (Varian, Palo Alto, CA, USA). HPLC-grade methanol and acetonitrile as well as disodium hydrogen phosphate, potassium dihydrogen phosphate, and potassium hydroxide were obtained from Sigma-Aldrich (St. Louis, MO, USA). Formic acid was purchased from Fluka (Buchs, Switzerland). Water was deionized with a purification system form Milli-Q reference (Billerica, MA, USA).