Magnetic beads coupled with sheep polyclonal antibodies antirabbit IgG (Dynabeads, Invitrogen) were used for co-immunoprecipitation studies. Cells were lysed in 50 mM Tris-HCl, pH 7.2, 2% CHAPS, protease inhibitor cocktail (Roche) and quantified with the BCA protein assay (Pierce). Proteins (500 µg) were diluted to 0.5 µg/µl with lysis buffer. Magnetic beads with coupled sheep antibodies antirabbit IgG (Dynabeads® M280; Invitrogen) were washed with 0.5% bovine serum albumin immonuglobulin-free (BSA Ig-free) in PBS to remove preservatives. Then 3 μg of rabbit polyclonal antihuman TDP-43 primary antibody (Proteintech; RRID: AB_2200505) or rabbit polyclonal antihuman Ran primary antibody (Abcam) was incubated with 20 μl of antirabbit IgG-conjugated Dynabeads for 2 h at 4°C in 0.1% BSA/PBS. Immunoprecipitation and analysis of the proteins are described in the Supplementary material .
Antihuman tdp 43 primary antibody
Manufactured by Proteintech
The Antihuman TDP-43 primary antibody is a laboratory tool used to detect the TDP-43 protein in human samples. TDP-43 is a DNA/RNA-binding protein involved in various cellular processes. This antibody can be used in techniques such as Western blotting, immunohistochemistry, and immunocytochemistry to identify and study the TDP-43 protein.
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2 protocols using antihuman tdp 43 primary antibody
1
Co-immunoprecipitation of TDP-43 and Ran
2
TDP-43 and Ran Protein Co-Immunoprecipitation
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Magnetic beads coupled with sheep polyclonal antibodies anti-rabbit IgG (Dynabeads, Invitrogen) were used for co-immunoprecipitation studies. Cells were lysed in 50 mM Tris-HCl, pH 7.2, 2% CHAPS, protease inhibitor cocktail (Roche) and quantified with the BCA protein assay (Pierce). Proteins (500 µg) were diluted to 0.5 µg/µL with lysis buffer. Magnetic beads with coupled sheep antibodies anti-rabbit IgG (Dynabeads® M280; Invitrogen) were washed with 0.5% bovine serum albumin immonuglobulin-free (BSA Ig-free) in PBS to remove preservatives. Three μg of rabbit polyclonal anti-human TDP-43 primary antibody (Proteintech; RRID: AB_2200505) or rabbit polyclonal anti-human Ran primary antibody (Abcam) was incubated with 20 μL of anti-rabbit IgGconjugated Dynabeads for 2h at 4°C in 0.1% BSA/PBS. Immunoprecipitation and analysis of the proteins are described in the Supplementary material.
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