The VEGF ELISA kit was from Thermo Scientific (Pittsburgh, PA). sVEGFR1 and rat VEGF ELISA kits were from R&D Systems (Minneapolis, MN). Recombinant human VEGF165 (a 165-amino acid splice variant of VEGF) was from EMD Chemicals (Gibbstown, NJ). Halt Protease inhibitor Single-Use Cocktail was obtained from Thermo Scientific (Pittsburgh, PA) and used at 1× or 3× the concentrations, as instructed by the vendor. Anti-human VEGFR1 antibodies (AF321 and BAF321) and normal goat immunoglobulin G (IgG) isotype control antibody were from R&D Systems (Minneapolis, MN). Recombinant human sVEGFR1 was from Cell Science (Canton, MA). Alexa Flour-conjugated Griffonia simplicifolia IB4 was obtained from Thermo Fisher Scientific (Waltham, MA), and anti-elastin was from Abcam (Cambridge, MA). Rat VEGF ELISA was obtained from R&D Systems (Minneapolis, MN).
Rat vegf elisa
Manufactured by R&D Systems
The Rat VEGF ELISA is an enzyme-linked immunosorbent assay designed for the quantitative measurement of rat vascular endothelial growth factor (VEGF) in cell culture supernatants, serum, and plasma samples.
Automatically generated - may contain errors
4 protocols using rat vegf elisa
1
Quantifying VEGF and sVEGFR1 Levels
2
Rat VEGF Serum Quantification Protocol
3
Serum VEGF Quantification in Rats
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
On p20, prior to euthanasia, blood samples were collected and placed at room temperature to allow blood to coagulate. After 2 h, clotted blood samples were spun down for 25 min at 2000×g, and the serum was collected. Serum samples were assayed as recommended using a rat VEGF ELISA from R&D systems (Minneapolis, MN).
4
Laser-induced CNV in Rat Model
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Laser-induced rupture of Bruch’s membrane was used to generate CNV in 6-week-old male Brown Norway rats. Rats were divided into treatment arms that included non-laser control, laser plus no injection, vehicle injection, and hUTC injection administered 7 days pre-laser. Choroidal tissues were dissected at 3 days post-laser treatment and homogenized, and VEGF protein was measured using a rat VEGF ELISA (R&D Systems, RRV00). VEGF protein levels were normalized to total protein in the choroidal tissues. Collected tissues included choroid, Bruch’s membrane, and RPE.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!