Uas gcamp5

Manufactured by Bloomington Drosophila Stock Center

The UAS-GCaMP5 is a genetic tool that can be used to visualize changes in calcium concentration within cells. It is a calcium-sensitive fluorescent protein that is expressed under the control of the GAL4/UAS system, allowing for targeted expression in specific cell types. The core function of UAS-GCaMP5 is to serve as a reporter for calcium dynamics, which can be used to study neural activity and other calcium-dependent cellular processes.

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Lab products found in correlation

Uas gcamp6m, by Bloomington Drosophila Stock Center (2 mentions) Uas rfp, by Bloomington Drosophila Stock Center (1 mentions) Dtrpa1 gal4, by Bloomington Drosophila Stock Center (1 mentions) Nsyb gal4, by Bloomington Drosophila Stock Center (1 mentions) R36g02 gal4, by Bloomington Drosophila Stock Center (1 mentions) Elav gal4, by Bloomington Drosophila Stock Center (1 mentions) Uas mcd8 gfp, by Bloomington Drosophila Stock Center (1 mentions)

2 protocols using uas gcamp5

Drosophila Strains for Neuroscience Research

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Drosophila melanogaster strains were raised on standard cornmeal/agar medium supplemented with dry yeast at 25 °C with a 12 hr light/dark cycle. The wild type stock was a w¹¹¹⁸strain. The following stocks were obtained from Bloomington Stock Center: w¹¹¹⁸;dTrpA1¹ (BL26504), dTrpA1 RNAi1 (BL31384), w*;dTrpA1¹, dTrpA1-Gal4 (BL36922), w*; dTrpA1-Gal4 (BL 27593), UAS-RFP (BL 27391), UAS-GCaMP5 (BL 42037), UAS-GCaMP6m (BL 42748). The Gr66a-IRES-GFP vector was kindly provided by Kristin Scott, the NSyB-Gal4;UAS-GCaMP3 was obtained by Patrick Verstreken. The lines Gr66aGal4, UAS-dTrpA1(A);dTrpA1^ins,dTrpA1GAL4, UAS-dTrpA1(B);dTrpA1^ins,dTrpA1Gal4 and the dTrpA1 RNAi 2 were kindly provided by the laboratory of Paul Garrity.

Soldano A., Alpizar Y.A., Boonen B., Franco L., López-Requena A., Liu G., Mora N., Yaksi E., Voets T., Vennekens R., Hassan B.A, & Talavera K. (2016). Gustatory-mediated avoidance of bacterial lipopolysaccharides via TRPA1 activation in Drosophila. eLife, 5, e13133.

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Fly Strain Characterization and Imaging

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The following fly strains were used in this study: yw (Bloomington stock no. 6598), nompC 1 and nompC 3 37 , Mhc 1 40 , GluRIIC 41 , 2-21-GAL4 31 , elav-GAL4 81 , nSyb-GAL4
(Bloomington stock center no. 58763), R36G02-GAL4 (Bloomington stock no. 49939) 25 , M-split-GAL4 (tsh-p65ADZp; VT002081-ZpGdbd), UAS-mCD8::GFP (Bloomington stock center no. 32186), UAS-GCaMP5 (Bloomington stock center no.
42038), UAS-GCaMP6m (Bloomington stock center no. 42750), UAS-CD4::GCaMP6f All experiments in this study were conducted with embryos and newly hatched larvae (ages ≤ 30 h AEL except for those shown in Figure 7E) at room temperature (25°C) unless otherwise noted. For accurate staging, eggs were collected for 20 minutes from parental flies raised in a cup at 25˚C covered with an yeast-pasted agar plate.

Zeng X., Komanome Y., Kawasaki T., Inada K., Jonaitis J., Pulver S.R., Kazama H, & Nose A. (2021). An electrically coupled pioneer circuit enables motor development via proprioceptive feedback in Drosophila embryos. Current biology : CB, 31(23).

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