Monoclonal anti flag clone m2

pIFNβ-Luc reporter plasmid and RIG-I expression plasmid were obtained from Dr Takashi Fujita (Kyoto University, Kyoto, Japan).^{15 (link)} Expression vectors for TBK1, IRF3 and TRAF3 were generous gifts from Dr Genhong Cheng (University of California, Los Angeles, CA, USA).^{16 (link),17 (link)} pISRE-Luc reporter construct was purchased from Clontech (Mountain View, CA, USA). MDA5, IKKε, MAVS and TANK expression vectors have been described.^{14 (link)}Monoclonal anti-FLAG (clone M2) and anti-α-tubulin (clone DM1A) antibodies were purchased from Sigma-Aldrich (St Louis, MO, USA). Anti-myc was bought from Santa Cruz (Dallas, TX, USA). Anti-V5 was from Life Technologies (Grand Island, NY, USA). Anti-GM130 was from BD Transduction (Lexington, KY, USA). Anti-calnexin was from Affinity Bioreagent (Golden, CO, USA).