Anti tgf β 5 and anti sox9 c 20 antibodies

All reagents were from Sigma (Munich, Germany) unless otherwise indicated. Recombinant FGF-2 and TGF-β3 were purchased at Peprotech (Hamburg, Germany). The dimethylmethylene blue dye was from Serva (Heidelberg, Germany). The anti-TGF-β (V) and anti-SOX9 (C-20) antibodies were from Santa Cruz Biotechnology (Heidelberg, Germany), the anti-type II collagen (II-II6B3) antibody from the NIH Hybridoma Bank (University of Iowa, Ames, IA, USA), the anti-type I collagen (AF-5610) antibody from Acris Antibodies (Hiddenhausen, Germany), and the anti-type X collagen (COL-10) antibody from Sigma. Biotinylated secondary antibodies and the ABC reagent were purchased at Vector Laboratories (Alexis Deutschland GmbH, Grünberg, Germany). The TGF-β enzyme-linked immunosorbent assay (hTGF-β1 Quantikine ELISA) was from R&D Systems (Wiesbaden, Germany).

All reagents were from Sigma‐Aldrich (Munich, Germany), unless otherwise identified. Recombinant TGF‐β (rTGF‐β) was purchased at Peprotech (Hamburg, Germany) and the dimethylmethylene blue dye at Serva (Heidelberg, Germany). The anti‐TGF‐β (V) and anti‐SOX9 (C‐20) antibodies were from Santa Cruz Biotechnology (Heidelberg, Germany), the anti‐type‐II collagen (II‐II6B3) antibody from the NIH Hybridoma Bank (University of Iowa, Ames, USA), the anti‐type‐I collagen (AF‐5610) antibody from Acris (Hiddenhausen, Germany) and the anti‐type‐X collagen (COL‐10) from Sigma‐Aldrich. Biotinylated secondary antibodies and the ABC reagent were obtained from Vector Laboratories (Alexis Deutschland GmbH, Grünberg, Germany). The hTGF‐β Quantikine ELISA was from R&D Systems (Wiesbaden, Germany) and the type‐II, ‐I and ‐X collagen ELISAs from from Antibodies‐Online (Aachen, Germany).