Pancreatic cancer (Panc1 and MiaPaCa2) and colon cancer (RKO, SW480) cell lines were purchased from American Type Culture Collection (Manassas, VA) and were authenticated on April 29, 2016 by Biosynthesis (Lewisville, TX). L3.6pL cells were kindly provided by Dr. I.J. Fidler (University of Texas MD Anderson Cancer Center, Houston, TX). Cells were maintained 37°C in the presence of 5% CO2 in Dulbecco’s Modified Eagle’s Medium/Ham’s F-12 medium with 10% fetal bovine serum with antibiotic. NR4A1 antibody was purchased from Novus Biologicals (Littleton, CO). Sp1 antibody and glutathione (GSH) reduced free acid were purchased from Millipore (Temecula, CA); β1-integrin, p300, Sp3 and Sp4 antibodies were purchased from Santa Cruz Biotech (Santa Cruz, CA). β-Actin antibody, Dulbecco’s Modified Eagle’s Medium, and 36% formaldehyde were purchased from Sigma-Aldrich (St. Louis, MO). Hematoxylin was purchased from Vector Laboratories (Burlingame, CA). FAK, p-FAK, α5-integrin antibodies and leptomycin were purchased from Cell Signaling Technologies (Manassas, VA).
Nr4a1 antibody
Manufactured by Novus Biologicals
The NR4A1 antibody is a laboratory reagent that can be used to detect and study the NR4A1 protein, also known as the nuclear receptor subfamily 4 group A member 1. NR4A1 is a transcription factor that plays a role in various cellular processes. The antibody can be used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to help researchers investigate the expression and localization of NR4A1 in different cell types and tissues.
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Lab products found in correlation
Hematoxylin, by Vector Laboratories (1 mentions)
Integrin β1, by Santa Cruz Biotechnology (1 mentions)
Sp1 antibody, by Merck Group (1 mentions)
Formaldehyde, by Merck Group (1 mentions)
Glutathione gsh reduced free acid, by Merck Group (1 mentions)
P fak, by Cell Signaling Technology (1 mentions)
β actin antibody, by Merck Group (1 mentions)
Dulbecco s modified eagle s medium, by Merck Group (1 mentions)
Panc 1, by American Type Culture Collection (1 mentions)
Mia paca 2, by American Type Culture Collection (1 mentions)
Cd8 isolation kit, by Miltenyi Biotec (1 mentions)
2 protocols using nr4a1 antibody
1
Molecular Mechanisms of Pancreatic and Colon Cancer
2
ChIP Assay of Activated CD8+ T Cells
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A total of 107 CD8+T cells, isolated from human PBMC using CD8 isolation kit (Miltenyi Biotec), was activated with plate-bound anti-CD3 (1 ug/ml) and anti-CD28 (1 ug/ml) in 96-well plates for 72 h. Chromatin immunoprecipitation (ChIP) assays were performed according to the protocol of the SimpleChIP® Plus Sonication Chromatin IP Kit (56,383). NR4A1 antibody used for ChIP was purchased from Novus Biologicals. DNA isolated was tested by real-time quantitative PCR. The enrichment of samples in chromatin was based on the calculation formula, △CT = CTChIP DNA – CTInput DNA. Specific information on antibodies was listed in Table. S1.
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