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St51011

Manufactured by R&D Systems
Sourced in Germany

The ST51011 is a laboratory equipment product designed for general use in research and development settings. It serves as a device for performing specific functions required in various scientific experiments and analyses. The core functionality of the ST51011 is to provide a tool for conducting relevant tasks, without further interpretation of its intended use or application.

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4 protocols using st51011

1

Serum Biomarker Profiling via ELISA

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Serum concentrations of interleukin (IL) 6 (Abcam, United States, ab46027), interferon (IFN) γ (Abcam, United States, ab46048), interferon-inducible protein (IP) 10 (Abcam, United States, ab173194), interferon α 1 (Abcam, United States, ab213479), IL-10 (Abcamn United States, ab100549), and HMGB-1 (IBL, Germany, ST51011), and the serum receptor for advanced glycation end-product (sRAGE, R&D, United States, DRG00) were detected via commercial ELISA kits following the instructions provided by the manufacturers (detailed in Supplementary materials).
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2

Quantifying Cytokine Secretion in Doxorubicin-Treated Cells

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Supernatants of cells treated with 5 µM or 0.2 µM doxorubicin or left untreated were harvested after 48 hours, and HMGB1, IL-6, TGF-β and VEGF were quantified using ELISA (IBL International GmbH, Cat#ST51011 and R&D Systems, Cat#DY406, DY1679, DY493).
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3

Quantitative Biomarker Profiling in Samples

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Commercially available ELISA kits were used to measure the concentrations or activity of HMGB1 (Shino Test Corporation, ST51011), DCN (R&D Systems, DY143, DY1060), IL6 (R&D Systems, M6000B, D6050), TNF (R&D Systems, MTA00B, DTA00D), NFKB (Active Motif, 43,296), MPO (Abcam, ab105136), LDH (Abcam, ab102526), trypsin (Abcam, ab102531), CTSB (Abcam, ab119585), and AMY (Abcam, ab102523) in the indicated samples.
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4

Cytokine Profiling of Doxorubicin-Treated Cells

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The supernatants of cells treated with 5 µM or 0.2 µM doxorubicin or left untreated were harvested after 48 hours, and HMGB1, IL-6, TGF-b and VEGF were quanti ed using ELISA (IBL International GmbH, Cat#ST51011 and R&D Systems, Cat#DY406, DY1679, DY493).
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