Sperm class analyzer version 6

Manufactured by Microptic

Sourced in Spain

The Sperm Class Analyzer version 6.3 is a laboratory equipment designed for the analysis and evaluation of sperm samples. It provides objective and quantitative data on various sperm parameters, including concentration, motility, and morphology.

Automatically generated - may contain errors

Lab products found in correlation

E200 microscope, by Nikon (4 mentions) Cfi plan achro 10x 0.25 ph1 bm, by Nikon (3 mentions) Aca1300 200um, by Basler (3 mentions) Aca1300 200μm, by Basler (1 mentions)

7 protocols using sperm class analyzer version 6

Computer-Assisted Sperm Motility Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Aliquots of sperm were placed in slide chamber (CellVision, 20 μm depth) and motility was examined on a 37°C stage of a Nikon E200 microscope under 10X phase contrast objective (CFI Plan Achro 10X/0.25 Ph1 BM, Nikon). Images were analyzed for spermatozoa by head tracing via computer-assisted sperm analysis (CASA, Sperm Class Analyzer version 6.3, Microptic, Barcelona, Spain). Images were recorded (40 frames at 50 fps) using CMOS video camera (Basler acA1300–200um, Basler AG, Ahrensburg, Germany) and analyzed by computer-assisted sperm analysis (CASA, Sperm Class Analyzer version 6.3, Microptic, Barcelona, Spain). Sperm motility (%) was quantified, and motion parameters including curvilinear velocity (VCL, in mm/s) and amplitude of lateral head displacement (ALH, in mM) were measured. Over 200 motile sperm were analyzed for each trial, with 3–4 biological replicates performed for each genotype, as denoted in figure legends.

Relovska S., Wang H., Zhang X., Fernández-Tussy P., Jeong K.J., Choi J., Suárez Y., McDonald J.G., Fernández-Hernando C, & Chung J.J. (2024). DHCR24-mediated sterol homeostasis during spermatogenesis is required for sperm mitochondrial sheath formation and impacts male fertility over time. bioRxiv.

+ Open protocol

+ Expand

Cauda Epididymal Sperm Analysis by CASA

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cauda epididymal sperm were collected by swim-out in HEPES buffered saline (HS) medium and analyzed by CASA (Sperm Class Analyzer version 6.3, Microptic). Over 200 motile sperm were analyzed for each trial. Three and two biological replicates were performed for the control and Ago2 cKO, respectively. Additional details are provided in the Supplemental Methods.

Griffin K.N., Walters B.W., Li H., Wang H., Biancon G., Tebaldi T., Kaya C.B., Kanyo J., Lam T.T., Cox A.L., Halene S., Chung J.J, & Lesch B.J. (2022). Widespread association of the Argonaute protein AGO2 with meiotic chromatin suggests a distinct nuclear function in mammalian male reproduction. Genome Research, 32(9), 1655-1668.

+ Open protocol

+ Expand

Computer-Assisted Sperm Motility Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Aliquots of sperm were placed in slide chamber (CellVision, 20 mm depth) and motility was examined on a 37 °C stage of a Nikon E200 microscope under 10× phase contrast objective (CFI Plan Achro 10×/0.25 Ph1 BM, Nikon). Images were recorded (40 frames at 50 fps) using CMOS video camera (Basler acA1300-200 μm, Basler AG) and analyzed by computer-assisted sperm analysis (CASA, Sperm Class Analyzer version 6.3, Microptic). Sperm total motility and hyperactivated motility was quantified simultaneously. Over 200 motile sperm were analyzed for each trial, at least three biological replicates were performed for each genotype. To track swimming trajectory, the sperm motility was videotaped at 50 fps. The images were analyzed using ImageJ software (v1.53) (70 (link)) by assembling overlays of the flagellar traces generated by hyperstacking binary images of 20 frames of 2 s movies coded in a gray intensity scale.

Wang H., Dou Q., Jeong K.J., Choi J., Gladyshev V.N, & Chung J.J. (2022). Redox regulation by TXNRD3 during epididymal maturation underlies capacitation-associated mitochondrial activity and sperm motility in mice. The Journal of Biological Chemistry, 298(7), 102077.

+ Open protocol

+ Expand

Sperm Motility Analysis Using CASA

Check if the same lab product or an alternative is used in the 5 most similar protocols

Aliquots of sperm were placed in slide chamber (CellVision, 20 μm depth) and motility was examined on a 37°C stage of a Nikon E200 microscope under 10× phase contrast objective (CFI Plan Achro 10X/0.25 Ph1 BM, Nikon). Images were recorded (40 frames at 50 fps) using CMOS video camera (Basler acA1300–200um, Basler AG, Ahrensburg, Germany) and analyzed by computer-assisted sperm analysis (CASA, Sperm Class Analyzer version 6.3, Microptic, Barcelona, Spain). Sperm motility (%) was quantified, and motion parameters including curvilinear velocity (VCL, in μm/s) and amplitude of lateral head displacement (ALH, in μM) were measured. For each time point, over 200 motile sperm were analyzed and for each experiment, replicate tests were performed using three (Efcab9^−/−) to four (WT and CatSper1^−/−) males from each genotype. For flagella waveform analysis, sperm loaded with BAPTA-AM or vehicle in M2 were imaged in the same medium. Sperm during recovery from BAPTA-AM were imaged in H-HTF. Movies were taken at 200 fps for 2 s and overlaid images from two beat cycles were generated by ImageJ.

Hwang J.Y., Mannowetz N., Zhang Y., Everley R.A., Gygi S.P., Bewersdorf J., Lishko P.V, & Chung J.J. (2019). Dual Sensing of Physiologic pH and Calcium by EFCAB9 Regulates Sperm Motility. Cell, 177(6), 1480-1494.e19.

+ Open protocol

+ Expand

Sperm Motility Quantification via CASA

Check if the same lab product or an alternative is used in the 5 most similar protocols

Aliquots of sperm were placed in slide chamber (CellVision, 20 mm depth) and motility was examined on a 37°C stage of a Nikon E200 microscope under 10X phase contrast objective (CFI Plan Achro 10X/0.25 Ph1 BM, Nikon). Images were recorded (40 frames at 50 fps) using CMOS video camera (Basler acA1300-200um, Basler AG, Ahrensburg, Germany) and analyzed by computer-assisted sperm analysis (CASA, Sperm Class Analyzer version 6.3, Microptic, Barcelona, Spain). Sperm total motility and hyperactivated motility was quantified simultaneously.
Over 200 motile sperm were analyzed for each trial, at least 3 biological replicates were performed for each genotype. To track swimming trajectory, the sperm motility was videotaped at 50 fps. The images were analyzed using Fiji software (Schindelin et al. 2012 ) by assembling overlays of the flagellar traces generated by hyperstacking binary images of 20 frames of 2 s movies coded in a gray intensity scale.

Wang H., Dou Q., Jung K.J., Choi J., Gladyshev V.N., & Chung J. (2021). Redox regulation by TXNRD3 during epididymal maturation underlies capacitation-associated mitochondrial activation and sperm motility in mice.

+ Open protocol

+ Expand

Sperm Motility and Viability Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

From each prepared sample, a 2.5 μl aliquot was placed in a 10 μm depth Leja chamber (Leja Products, Nieuw Vennep, the Netherlands) which was then placed on a heated plate at 37°C for 5 min before measuring concentration and motility using Sperm Class Analyzer, version 6 (Microptic SL, Barcelona, Spain) attached to a Microtec LM-2 Microscope (Mazurek Optical Services Ltd, Southam, UK) via a Basler acA1300-200uc camera (Basler AG, Ahrensburg, Germany). Since PBS does not contain any metabolites, sperm suspended in it generally swim poorly and so this process was repeated with a 20 μl aliquot of prepared sperm diluted 1 in 2 in PureSperm Wash (Nidacon, Gothenburg, Sweden) to assess the ability of the prepared sperm to swim when placed in a conventional medium. In addition, sperm vitality of each prepared sample was assessed using the LIVE/DEAD™ sperm viability kit (Fisher Scientific, Loughborough, UK), counting two replicates of at least 200 sperm as either alive (green) or dead (red) in order to establish the percentage of viable sperm.

Calvert S.J., Reynolds S., Paley M.N., Walters S.J, & Pacey A.A. (2018). Probing human sperm metabolism using 13C-magnetic resonance spectroscopy. Molecular Human Reproduction, 25(1), 30-41.

+ Open protocol

+ Expand

Automated Sperm Analysis System

Check if the same lab product or an alternative is used in the 5 most similar protocols

A computer-aided sperm analyzer system (Sperm Class Analyzer version 6; Microptic) was used to assess motility and the motility indices. In this study, 5-µL samples from each group were placed in a preheated Makler chamber (Proiser), and general motility, progressive movement and motor indices, average path velocity (μm/sec), curvilinear velocity (μm/sec), straight-line velocity (μm/sec), mean linearity (μm/sec), straightness (μm/sec), amplitude of lateral head displacement (μm), and beat cross frequency (Hz) were assessed for 500 sperm.

Farazmand T., Mansouri F., Koohestanidehaghi Y, & Shahandeh E. (2023). Human sperm parameter improvement associated with Ceratonia siliqua extract as a cryopreservation supplement after vitrification. Clinical and Experimental Reproductive Medicine, 50(2), 86-93.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!