Log In Sign up
The largest database of trusted experimental protocols

Ifn γ il 10 secretion assay detection kit

Manufactured by Miltenyi Biotec
Visit Supplier

The IFN-γ/IL-10 Secretion Assay Detection Kit is a laboratory product designed for the simultaneous detection and analysis of interferon-gamma (IFN-γ) and interleukin-10 (IL-10) secretion from stimulated cells. The kit provides the necessary reagents and components to perform this specific cell-based assay.

Automatically generated - may contain errors

Lab products found in correlation

Fcap array v3, by BD (2 mentions) Human mouse th1 th2 th17 cytometric bead array, by BD (2 mentions) Attune nxt flow cytometer, by BioLegend (2 mentions) Legendplex human inflammation panel 1 13 plex, by BioLegend (2 mentions) Facscanto 2, by BD (2 mentions)

2 protocols using ifn γ il 10 secretion assay detection kit

1

Cytokine Profiling by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols
Supernatants from 96-well plates were aliquoted and stored at -20C. Cytokines were quantified by the Human/Mouse Th1/Th2/Th17 Cytometric Bead Array (CBA) (BD Biosciences) using a FACSCanto II (BD Biosciences), analyzed by FCAP Array v3.0 (BD Biosciences). Alternatively, we used the LEGENDplex Human Inflammation Panel 1 (13plex) (740808, BioLegend) with the Attune NxT Flow Cytometer. Cytokine assays analyzed by FlowJo/v9.
For intracellular cytokine detection, the IFN-γ/IL-10 Secretion Assay Detection Kit (Miltenyi, #130-090-761) was used on live cells after 72h.
Chauss D., Freiwald T., McGregor R., Yan B., Wang L., Nova-Lamperti E., Kumar D., Zhang Z., Teague H., West E.E., Vannella K.M., Ramos-Benitez M.J., Bibby J., Kelly A., Malik A., Freeman A.F., Schwartz D.M., Portilla D., Chertow D.S., John S., Lavender P., Kemper C., Lombardi G., Mehta N.N., Cooper N., Lionakis M.S., Laurence A., Kazemian M, & Afzali B. (2021). Autocrine Vitamin D-signaling switches off pro-inflammatory programs of Th1 cells. Nature immunology, 23(1), 62-74.
+ Open protocol
+ Expand
2

Cytokine Profiling by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols
Supernatants from 96-well plates were aliquoted and stored at -20C. Cytokines were quantified by the Human/Mouse Th1/Th2/Th17 Cytometric Bead Array (CBA) (BD Biosciences) using a FACSCanto II (BD Biosciences), analyzed by FCAP Array v3.0 (BD Biosciences). Alternatively, we used the LEGENDplex Human Inflammation Panel 1 (13plex) (740808, BioLegend) with the Attune NxT Flow Cytometer. Cytokine assays analyzed by FlowJo/v9.
For intracellular cytokine detection, the IFN-γ/IL-10 Secretion Assay Detection Kit (Miltenyi, #130-090-761) was used on live cells after 72h.
Chauss D., Freiwald T., McGregor R., Yan B., Wang L., Nova-Lamperti E., Kumar D., Zhang Z., Teague H., West E.E., Vannella K.M., Ramos-Benitez M.J., Bibby J., Kelly A., Malik A., Freeman A.F., Schwartz D.M., Portilla D., Chertow D.S., John S., Lavender P., Kemper C., Lombardi G., Mehta N.N., Cooper N., Lionakis M.S., Laurence A., Kazemian M, & Afzali B. (2021). Autocrine Vitamin D-signaling switches off pro-inflammatory programs of Th1 cells. Nature immunology, 23(1), 62-74.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!