Mouse ribopure

Manufactured by Thermo Fisher Scientific

The Mouse RiboPure is a laboratory equipment designed for the purification of total RNA from mouse tissue samples. It utilizes a specialized chemical extraction method to isolate high-quality RNA from a variety of mouse tissues, including liver, spleen, and brain. The equipment is intended to provide researchers with a reliable and efficient tool for RNA extraction, which is a crucial step in various molecular biology and genetic research applications.

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Lab products found in correlation

Nanodrop spectrophotometer, by Thermo Fisher Scientific (4 mentions) Axon genepix 4000b scanner, by Molecular Devices (2 mentions) Globinclear, by Thermo Fisher Scientific (2 mentions) Messageamp premier rna amplification kit, by Thermo Fisher Scientific (2 mentions) Agilent bioanalyzer, by Agilent Technologies (1 mentions)

Spelling variants of this product

Mouse RiboPure Blood RNA Isolation Kit (23 citations)

2 protocols using mouse ribopure

Murine Whole Blood RNA Isolation and Microarray

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Whole blood RNA isolation and β-globin reduction were carried out on 30 samples (10 from each experimental group) using the manufacturer’s protocol (Mouse RiboPure and GLOBINclear, Ambion). The amount and purity of RNA yield was analyzed using the NanoDrop spectrophotometer (Thermo Fischer Scientific, Waltham, MA, USA) and the integrity was analyzed using the Agilent Bioanalyzer. RNA from 28 of the 30 samples that met quality control checks (260/280 ratio > 1.8, 260/230 ratio > 1.0, and RNA integrity number > 7) were used for microarray analysis. RNA was amplified and biotin-labeled using MessageAmp Premier RNA Amplification kit (Ambion) according to standard protocols at the Duke University Microarray Core facility. The Duke University Microarray Core performed amplification and hybridization onto Affymetrix murine 430A2.0 microarrays. Probe intensities were detected using Axon GenePix 4000B Scanner (Molecular Devices, San Jose, CA, USA). Image files were generated using Affymetrix GeneChip Command Console software.

Holcomb Z.E., Steinbrink J.M., Zaas A.K., Betancourt M., Tenor J.L., Toffaletti D.L., Alspaugh J.A., Perfect J.R, & McClain M.T. (2022). Transcriptional Profiles Elucidate Differential Host Responses to Infection with Cryptococcus neoformans and Cryptococcus gattii. Journal of Fungi, 8(5), 430.

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Murine Whole Blood RNA Isolation and Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Whole blood RNA isolation and β-globin reduction were carried out on 60 mice using the manufacturer’s protocol (Mouse RiboPure and GLOBINclear, Ambion). The amount and purity of RNA yield was analyzed using a NanoDrop spectrophotometer (Thermo Fischer Scientific) and the integrity was analyzed using an Agilent Bioanalyzer. RNA from the 60 samples that met quality control checks (260/280 ratio> 1.8, 260/230 ratio >1.0, and RNA integrity number >7) were used for microarray analysis. RNA was amplified and biotin-labeled using MessageAmp Premier RNA Amplification kit (Ambion) according to standard protocols at the Duke University Microarray Core facility. The Duke University Microarray Core performed amplification and hybridization onto Affymetrix murine 430A2.0 microarrays. Probe intensities were detected using Axon GenePix 4000B Scanner (Molecular Devices). Image files were generated using Affymetrix GeneChip Command Console software.

Steinbrink J.M., Zaas A.K., Betancourt M., Modliszewski J.L., Corcoran D.L, & McClain M.T. (2020). A Transcriptional Signature Accurately Identifies Aspergillus Infection Across Healthy and Immunosuppressed States. Translational research : the journal of laboratory and clinical medicine, 219, 1-12.

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