Transwell chamber system with 8-μm pore polycarbonate filter inserts (CoSTAR, Acton, MA) was used for determination of invasive potential. The lower and upper sides of the filter were coated with gelatin and matrigel, respectively. Trypsinized cells (5 × 103) in the serum-free RPMI 1640 medium containing 0.1% bovine serum albumin were added to each upper chamber of the transwell. RPMI 1640 medium containing 10% fetal bovine serum was placed in the lower chamber and cells were incubated at 37°C for 16 h. For determination of migration potential, the lower sides of the filters were coated with gelatin.
Transwell chamber system with 8 μm pore polycarbonate filter inserts
Manufactured by Corning
Sourced in United States
The Transwell chamber system with 8-μm pore polycarbonate filter inserts is a laboratory equipment product designed for cell culture applications. The system consists of a chamber with a permeable membrane insert, allowing for the separation and study of cells in a controlled environment.
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2 protocols using transwell chamber system with 8 μm pore polycarbonate filter inserts
1
Transwell Invasion and Migration Assay
2
Transwell Invasion Assay Protocol
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A transwell chamber system with 8-μm pore polycarbonate filter inserts (CoSTAR, Acton, MA, United States) was employed. Trypsinized cells (5 × 103) in the serum-free RPMI 1,640 medium containing 0.1% bovine serum albumin were added to each upper chamber of the transwell. RPMI 1,640 medium supplemented with 10% fetal bovine serum was placed in the lower chamber and cells were incubated at 37°C for 16 h. The invaded cells were stained and counted as described (Kim et al., 2017a (link)). Differences were considered significant when p < 0.05.
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