Human normal thyroid cell line Nthy-ori 3-1; PTC cell line TPC-1, BCPAP and K1; human embryonic kidney 293T (HEK293T) cell line was purchased from Shanghai Cell Bank, Chinese Academy of Sciences. Nthy-ori 3-1, TPC-1, BCPAP cell lines were reared with RPMI-1640 complete medium (Meilunbio, Dalian, China), while 293T and K1 were reared in DMEM-H complete medium (Meilunbio, Dalian, China). All media contained 10% fetal bovine serum (FBS) (ExCell Bio, Shanghai, China) and 1% penicillin-streptomycin (Beyotime, Shanghai, China), and the cell culture conditions were 37 °C, 5% carbon dioxide.
Rpmi 1640 complete medium
Manufactured by Meilun
Sourced in China
RPMI-1640 complete medium is a culture medium commonly used for the in vitro cultivation of a variety of cells, including mammalian and human cell lines. It provides the necessary nutrients and growth factors to support the growth and maintenance of these cell types.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using rpmi 1640 complete medium
1
Cell Culture Protocols for Thyroid and Kidney Cell Lines
2
Culturing Human Gastric Cell Lines
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Human gastric mucosa epithelial cells GES1, human gastric cancer cells MGC-803, AGS, MKN-45 and HGC27 cells were purchased from Beina Bio (BNCC, Beijing, China). MGC-803, MKN-45 and HGC27 cells were reared in RPMI-1640 complete medium (Meilunbio, Dalian, China). AGS cells were fed in F-12 complete medium (Meilunbio, Dalian, China) and GES1 in DMEM-H complete medium (Meilunbio, Dalian, China). All media contained 10% fetal bovine serum (FBS) (Gibco, New York, NY, USA) and 1% penicillin-streptomycin (Beyotime, Shanghai, China), and all cells were grown in an incubator at 37 °C and 5% CO2.
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