3 cyano n 1 3 diphenyl 1h pyrazol 5 yl benzamide cdppb

Manufactured by Bio-Techne

3-cyano-N-(1,3-diphenyl-1H-pyrazol-5-yl)benzamide (CDPPB) is a chemical compound. It has the molecular formula C25H18N4O.

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Lab products found in correlation

D ap5, by Merck Group (1 mentions) D 2 amino 5 phosphonopentanoic acid d ap5, by Bio-Techne (1 mentions) Bicuculline bic, by Bio-Techne (1 mentions) Cycloheximide (chx), by Merck Group (1 mentions) Mtor inhibitor rapamycin, by LC Laboratories (1 mentions) Cdppb, by Bio-Techne (1 mentions) Cocaine hydrochloride, by Mallinckrodt (1 mentions)

Spelling variants of this product

CDPPB (12 citations) Benzamide (2 citations)

2 protocols using 3 cyano n 1 3 diphenyl 1h pyrazol 5 yl benzamide cdppb

Pharmacological Modulation of Synaptic Plasticity

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NMDAR antagonist D-(–)-2-amino-5-phosphonopentanoic acid (D-AP5, Cat#-0106), GABA type A receptor (GABA_AR) antagonist bicuculline (BIC; Cat#-0130), and positive mGluR5 allosteric modulator 3-cyano-N-(1,3-diphenyl-1H-pyrazol-5-yl)benzamide (CDPPB, Cat#-3235) were purchased from Tocris Bioscience (Minneapolis, MN). Negative mGluR5 allosteric modulator 2-chloro-4-([2,5-dimethyl-1-(4-[trifluoromethoxy]-phenyl)-1H-imidazol-4-yl]ethynyl)pyridine (CTEP,^{23 (link)} Cat#-B1633) was purchased from ApexBio Technology (Boston, MA), and mTOR inhibitor rapamycin (Cat#-R-5000) was purchased from LC Laboratories (Woburn, MA). Protein synthesis inhibitor cycloheximide (CHX, Cat#-01810) was purchased from Sigma (St Louis, MO).
The half-maximal inhibitory concentration of D-AP5 to inhibit NMDAR-mediated LTD is 0.45μM,^{24 (link)} and we used 50μM D-AP5.^{22 (link),25 (link)} We used 25μM BIC, at which concentration it reliably blocks GABA_ARs.^{26 (link)}

Sun Y., Lipton J.O., Boyle L.M., Madsen J.R., Goldenberg M.C., Pascual-Leone A., Sahin M, & Rotenberg A. (2016). Direct Current Stimulation Induces mGluR5-Dependent Neocortical Plasticity. Annals of neurology, 80(2), 233-246.

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Estradiol and Cocaine Reinforcement in Ovariectomized Rats

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Estradiol (17β-estradiol; E2758, Sigma-Aldrich, St. Louis, MO) was dissolved in cottonseed oil to a final concentration of 2 μg/0.1 ml and was injected s.c. at a volume of 0.1 ml. The mGluR5 antagonist MPEP (1212, Tocris, Minneapolis, MN) was dissolved in sterile saline (1 mg/ml/kg) and injected i.p. This dose of MPEP has been shown to block estradiol-induced changes in dendritic spines within the NAc (Peterson et al., 2014 (link)), as well as estradiol enhancement of behavioral sensitization (Sircar and Kim, 1999 (link); Martinez et al., 2014 (link)), in OVX female rats. The mGluR5-positive allosteric modulator 3-cyano-N-(1,3-diphenyl-1H-pyrazol-5-yl)benzamide (CDPPB; 3235, Tocris) was dissolved in 10% Tween-80 (low CDPPB, 10 mg/ml/kg; high CDPPB, 25 mg/2 ml/kg) and injected i.p. The 10 mg/ml/kg dose of CDPPB mimics the effects of estradiol on dendritic spine density in the NAc in OVX females (Gross et al., 2016 (link)). Cocaine (cocaine hydrochloride; 0406-1520-53, Mallinckrodt, St. Louis, MO) was dissolved in sterile PBS (9.3 mg/ml) and infused i.v. (1.5 mg/kg/infusion). Previous work has shown that under extended access conditions, estradiol treatment enhances cocaine intake in OVX females self-administering at this dose (Ramôa et al., 2013 (link)).

Martinez L.A., Gross K.S., Himmler B.T., Emmitt N.L., Peterson B.M., Zlebnik N.E., Foster Olive M., Carroll M.E., Meisel R.L, & Mermelstein P.G. (2016). Estradiol Facilitation of Cocaine Self-Administration in Female Rats Requires Activation of mGluR5. eNeuro, 3(5), ENEURO.0140-16.2016.

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