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Mca5776ga

Manufactured by Bio-Rad
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Sourced in United Kingdom, United States

The MCA5776GA is a laboratory equipment product manufactured by Bio-Rad. It serves as a core function for laboratory applications, but a detailed description cannot be provided while maintaining an unbiased and factual approach.

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Lab products found in correlation

Pan actin, by Cell Signaling Technology (3 mentions) β actin, by Bio-Rad (3 mentions) Mca5775ga, by Bio-Rad (3 mentions) α tubulin, by Cell Signaling Technology (2 mentions) γ actin, by Bio-Rad (2 mentions) Goat anti rabbit igg, by Jackson ImmunoResearch (2 mentions) Cyclin e2, by Cell Signaling Technology (1 mentions) Cyclin b1, by Cell Signaling Technology (1 mentions) Cyclin d1, by Cell Signaling Technology (1 mentions) Cyclin d3, by Cell Signaling Technology (1 mentions) Cyclin a2, by Cell Signaling Technology (1 mentions) Igg2b, by Jackson ImmunoResearch (1 mentions) Cyclin h, by Cell Signaling Technology (1 mentions) Alexa fluor 647 conjugated goat anti mouse igg, by Jackson ImmunoResearch (1 mentions) P p44 42 t202 y204, by Cell Signaling Technology (1 mentions) Cyclin e1, by Cell Signaling Technology (1 mentions) Erk1 2, by Cell Signaling Technology (1 mentions) Tubulin, by Merck Group (1 mentions) α tubulin, by Bio-Rad (1 mentions) Cenp a, by Cell Signaling Technology (1 mentions)

3 protocols using mca5776ga

1

Antibody Panel for Cell Cycle Analysis

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Mouse monoclonal antibodies to: β-actin (MCA5775GA, AbD Serotec); γ-actin (MCA5776GA, AbD Serotec); pan actin (4968, Cell Signaling); α-tubulin (2144, Cell Signaling); p-p44/42 (T202/Y204) (4370, Cell Signaling); ERK1/2 (4695, Cell Signaling); cyclin A2 (4656, Cell Signaling); cyclin D3 (2936, Cell Signaling); cyclin E1 (4129, Cell Signaling). Rabbit monoclonal antibodies: cyclin B1 (4138, Cell Signaling); cyclin D1 (2978, Cell Signaling); cyclin E2 (4132, Cell Signaling); cyclin H (2927, Cell Signaling).
The following secondary antibodies were used: AlexaFluor488-, AlexaFluor594, AlexaFluor647- conjugated goat anti-mouse IgG, IgG1, IgG2b and goat anti-rabbit IgG (Jackson ImmunoResearch Laboratories. Inc).
Dugina V., Shagieva G., Khromova N, & Kopnin P. (2018). Divergent impact of actin isoforms on cell cycle regulation. Cell Cycle, 17(23), 2610-2621.
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2

Immunofluorescence Cytoskeleton Protein Labeling

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Mouse monoclonal antibodies to: β-actin (MCA5775GA, AbD Serotec); γ-actin (MCA5776GA, AbD Serotec); pan actin (4968, Cell Signaling); α-tubulin (2144, Cell Signaling); rat monoclonal antibodies to α-tubulin (Serotec); rabbit polyclonal antibodies to EB1 (Gen Script) and tubulin (Sigma-Aldrich).
The following secondary antibodies were used: AlexaFluor488-, AlexaFluor594-, AlexaFluor647-, Cy5-conjugated goat anti-mouse IgG1, IgG2b, IgG, goat anti-rabbit IgG and goat anti-rat IgG (Southern Biotechnology, Associates Inc., Birmingham, AL; Jackson; Life technologies).
Dugina V., Alieva I., Khromova N., Kireev I., Gunning P.W, & Kopnin P. (2016). Interaction of microtubules with the actin cytoskeleton via cross-talk of EB1-containing +TIPs and γ-actin in epithelial cells. Oncotarget, 7(45), 72699-72715.
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3

Immunofluorescence Staining of Cytoskeletal and Chromatin Proteins

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Mouse monoclonal antibodies to: β-actin (MCA5775GA, AbD Serotec); γ-actin (MCA5776GA, AbD Serotec, Raleigh, NC, USA); pan actin (4968, Cell Signaling) were used. Rabbit polyclonal antibodies to CENP-A (2186, Cell Signaling) were used. Rabbit monoclonal antibodies to: Phospho-Histone H3 (Ser10, 3377, Cell Signaling); Histone H3 (4499, Cell Signaling) were used.
The following secondary antibodies were used: AlexaFluor488-, AlexaFluor594- conjugated goat anti-mouse IgG, IgG1, IgG2b and goat anti-rabbit IgG (Jackson ImmunoResearch Laboratories Inc, West Grove, PA, USA).
Dugina V., Shagieva G., Novikova M., Lavrushkina S., Sokova O., Kireev I, & Kopnin P. (2021). Impaired Expression of Cytoplasmic Actins Leads to Chromosomal Instability of MDA-MB-231 Basal-Like Mammary Gland Cancer Cell Line. Molecules, 26(8), 2151.
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