Taxol

Methanol was of mass spectrometry (MS) grade from Merck (Darmstadt, Germany). Formic acid (MS grade), hexafluoro-2-propanol (HFIP; ≥99.0%), trimethylamine (TEA; ≥99.5%), and guanidinium thiocyanate were purchased from Sigma (USA). MicroRNA marker and low-range ssRNA ladder were purchased from New England Biolabs (USA). Diethylpyrocarbonate (DEPC)-treated water, polyacrylamide containing a ratio of acrylamide/bis (19:1, w/w) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Thermo (USA). RPMI 1640 medium, MEM medium, DMEM, fetal bovine serum (FBS), penicillin, and streptomycin were purchased from Gibco (New Zealand). Taxol (purity ≥ 98%) was purchased from AdooQ bioscience (Nanjing, China). Deionized water was prepared by a Millipore Milli-Q Plus system (Millipore, USA). All reagents used were of analytical grade.

Methanol and acetonitrile were of MS grade from Merck (Darmstadt, Germany). Formic acid (MS grade), hexafluoro-2-propanol (HFIP, ≥99.0%), trimethylamine (TEA, ≥99.5%), Transcriptor Universal cDNA Master kit, protease Inhibitor Cocktail, phosphatase inhibitor, phosphodiesterase I and II from Crotalus adamanteus venom were purchased from Sigma-Aldrich (St. Louis, MO, USA). TRIzol reagent, mirVana™ miRNA isolation kit, Pierce™ Streptavidin-coated magnetic beads, RNase I and RNase T1 were purchased from Thermo Fisher Scientific (Waltham, MA, USA). A low-range ssRNA ladder and shrimp alkaline phosphatase were purchased from New England Biolabs (Beverly, MA, USA). RPMI-1640 medium, fetal bovine serum (FBS), penicillin, streptomycin and puromycin were obtained by Gibco (Carlsbad, CA, USA). RNase A was purchased from Takara Biotechnology (Dalian, China). Reference standards, 4-demethylwyosine (imG-14), wyosine (imG) and isowyosine (imG2), were provided by 9dingchem Co., Ltd (Shanghai, China). The purity was more than 98%. Taxol (purity ≥ 98%) was supplied by AdooQ bioscience (Nanjing, China). Ultrapure water (18.25 MΩ·cm) was produced using a Milli-Q system from Millipore (Millipore, Bedford, MA, USA).