Authenticated human Merkel cell carcinoma cell lines (MKL-1 and WaGa) were
obtained courtesy of Dr. James DeCaprio at the Dana-Farber Cancer Institute, Boston,
MA[13 (link)] and were cultured fewer than 6 months in
RPMI 1640 medium supplemented with 20% (v/v) FBS and 1% (v/v)
penicillin/streptomycin (Gibco, Life Technologies, Grand Island, NY). Authenticated human
umbilical vein endothelial cells (HUVEC) were obtained from the American Type Culture
Collection (ATCC) and cultured in M199 medium supplemented with 10% (v/v) FBS,
1% (v/v) penicillin/streptomycin (Gibco, Life Technologies), 100µg/ml
(wt/v) endothelial cell growth supplement (Biomedical Technologies, Ward Hill, MA),
100µg/ml (wt/v) heparin, 100nM (v/v) hydrocortisone and 100nM (v/v) ascorbic acid
in fibronectin-coated (20µg/ml) flasks (Sigma-Aldrich, St. Louis, MO).
obtained courtesy of Dr. James DeCaprio at the Dana-Farber Cancer Institute, Boston,
MA[13 (link)] and were cultured fewer than 6 months in
RPMI 1640 medium supplemented with 20% (v/v) FBS and 1% (v/v)
penicillin/streptomycin (Gibco, Life Technologies, Grand Island, NY). Authenticated human
umbilical vein endothelial cells (HUVEC) were obtained from the American Type Culture
Collection (ATCC) and cultured in M199 medium supplemented with 10% (v/v) FBS,
1% (v/v) penicillin/streptomycin (Gibco, Life Technologies), 100µg/ml
(wt/v) endothelial cell growth supplement (Biomedical Technologies, Ward Hill, MA),
100µg/ml (wt/v) heparin, 100nM (v/v) hydrocortisone and 100nM (v/v) ascorbic acid
in fibronectin-coated (20µg/ml) flasks (Sigma-Aldrich, St. Louis, MO).