SC-neuron cultures growing on PLL-laminin-coated glass cover slips were fixed overnight in 2% glutaraldehyde-100 mM sucrose and then rinsed in 0.15 M phosphate buffer before post-fixing for 1 h with 2% OsO4. Subsequently, cells were rinsed, dehydrated in graded ethanol solutions and Embedded in Embed (Electron Microscopy Sciences, Hatfield, PA). Thin sections obtained with a Leica Ultracut E microtome were stained with uranylacetate/lead citrate for examination in a Philips CM-10 transmission electron microscope.
Ultracut e microtome
Manufactured by Philips
The Ultracut E microtome is a precision instrument designed for the sectioning of materials for microscopic examination. It features an advanced cutting mechanism that allows for the preparation of high-quality thin sections with consistent thickness. The Ultracut E is a versatile tool suitable for a variety of applications in fields such as materials science, biology, and medical research.
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Lab products found in correlation
2 protocols using ultracut e microtome
1
Ultrastructural Analysis of Neurons
2
Oocyte Ultrastructural Imaging
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Oocytes were fixed with 2% glutaraldehyde in sodium cacodylate buffer with the addition of 1,600 ppm ruthenium hexamine trichloride. Samples were washed and postfixed with 1% Osmium tetroxide, including 1,600 ppm ruthenium hexamine trichloride, followed by en bloc staining with 1% uranyl acetate and dehydration through a graded progressive series of acetone concentrations, and embedded overnight in EMBed812 resin before polymerization, then erially sectioned at 100 nm using a Reichart Ultracut E microtome; ribbons were collected and viewed on a Philips CM100.
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