Seo sem inspect s50 b

Immersion test was conducted in a simulated body fluid (SBF) which has similar ion concentrations to human blood plasma (Table 2). All the samples were immersed separately in 50 mL of SBF at 37 °C (initial pH value of 7.4 ± 0.02). The immersed samples were placed in fresh SBF without agitation every 2 days to keep a stable chemical composition and pH value of the SBF. A wide range of immersion times including 1, 3, 7 and 63 days were selected. The degradation rates of the samples were evaluated by weight loss measurements and surface morphology analyzes. The samples were weighed before the test and after each period of immersion.
Scanning electron microscope SEO-SEM Inspect S50-B (FEI, Brno, Czech Republic) was used to describe the surface characteristics after the immersion test. The chemical composition of the surface before and during the examination was evaluated by an energy-dispersive X-ray spectrometer AZtecOne with X-MaxN20 detector (Oxford Instruments, Abingdon, Oxfordshire, UK) attached to the SEM.

The samples were sputter-coated with a thin layer of silver (30–50 nm) under vacuum setup VUP-5M (SELMI, Sumy, Ukraine) and visualized with SEO-SEM Inspect S50-B (FEI, Brno, Czech Republic) scanning electron microscope. Assessment of nanofiber morphology and mesh pore analysis were based on SEM images, using DiameterJ plugin 1.018w for Fiji (distribution ImageJ 1.51w, University of Wisconsin, Madison, WI, USA).