Protein was extracted using a Total Protein Extraction kit (KeyGEN Biotech, Nanjing, China), and the protein concentration was quantified using a BCA Protein Assay kit (KeyGEN Biotech). Each sample containing an equivalent amount of protein (20 µg) was separated by 10% SDS‐PAGE and transferred to polyvinylidene difluoride (PVDF) membranes. After blocking with 5% skim milk powder for 1 hour at room temperature, the PVDF membranes were incubated overnight at 4°C with a rabbit antibody against E2F3 (1:1000; Affinity Biosciences, New Jersey, USA) and a rabbit monoclonal antibody against GAPDH (1:1000) (Beyotime Biotechnology, Beijing, China). Then, the membranes were washed three times with TBS‐T buffer, which was followed by incubation with a goat anti‐rabbit secondary antibody (1:1000; Beyotime Biotechnology) for 1 hour at room temperature. Immunoreactive proteins were detected using an ECL Reagent (Affinity) and an automatic chemiluminescence image analysis system (Tanon 5200, Shanghai, China).
Rabbit antibody against e2f3
Manufactured by Affinity Biosciences
Sourced in United States
This rabbit antibody is specific to the E2F3 protein. E2F3 is a transcription factor that plays a key role in cell cycle regulation. This antibody can be used to detect and study the E2F3 protein in research applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using rabbit antibody against e2f3
1
Quantification of E2F3 Protein Expression
2
Protein Extraction and Western Blot
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Protein was extracted using a Total Protein Extraction kit (KeyGEN Biotech, Nanjing, China), and the protein concentration was quantified using a BCA Protein Assay kit (KeyGEN Biotech, Nanjing, China). Each sample containing an equivalent amount of protein (20 µg) was separated by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene difluoride (PVDF) membranes. After blocking with 5% skim milk powder for one hour at room temperature, the PVDF membranes were incubated overnight at 4°C with a rabbit antibody against E2F3 (1:1000, Affinity, USA) and a rabbit monoclonal antibody against GAPDH (1:1000) (Beyotime Biotechnology, Beijing, China). Then, the membranes were washed 3 times with TBS-T buffer, which was followed by incubation with a goat anti-rabbit secondary antibody (1:1000, Beyotime Biotechnology, Beijing, China) for one hour at room temperature. Immunoreactive proteins were detected using an ECL Reagent (Affinity, USA) and an automatic chemiluminescence image analysis system (Tanon 5200).
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