The following antibodies were used in flow cytometry: PE anti-mouse H-2Kb/H-2Db, PE anti-mouse IFNγR β chain (Biolegend, San Diego, CA, USA), PE anti-mouse CD119 (IFNγ Receptor 1) (eBioscience, San Diego, CA, USA). Antibodies used in western blotting analysis were: iNOS, pTyr701-Stat1, total Stat1, total Stat2, pSer536-p65, total p65 pSer32-IκBα, total IκBα, β-actin, GAPDH (Cell Signaling Technology, Danvers, MA, USA); pTyr690-Stat2 (Abcam, Cambridge, MA, USA); LaminB (Epitomics, Burlingame, CA, USA). Recombinant mouse IFNα, IFNβ, IFNγ and TNFα were from R&D Systems (Minneapolis, MN, USA). L-NMMA was from Sigma-Aldrich (St. Louis, MO, USA).
Ptyr690 stat2
Manufactured by Abcam
Sourced in United States
PTyr690-Stat2 is a phospho-specific antibody that recognizes the phosphorylated tyrosine 690 residue of Signal Transducer and Activator of Transcription 2 (STAT2) protein. This antibody can be used in various immunoassay applications to detect and quantify the phosphorylation of STAT2 at this specific site.
Automatically generated - may contain errors
Lab products found in correlation
L nmma, by Merck Group (2 mentions)
Total stat1, by Cell Signaling Technology (2 mentions)
Pe anti mouse h 2kb h 2db, by BioLegend (2 mentions)
Ptyr701 stat1, by Cell Signaling Technology (2 mentions)
Total stat2, by Cell Signaling Technology (2 mentions)
Total iκbα, by Cell Signaling Technology (2 mentions)
Pe anti mouse ifnγr β chain, by BioLegend (2 mentions)
Total p65 pser32 iκbα, by Cell Signaling Technology (2 mentions)
Lamin b, by Abcam (2 mentions)
Recombinant mouse ifnα, by R&D Systems (2 mentions)
P p65 ser536, by Cell Signaling Technology (2 mentions)
Gapdh, by Cell Signaling Technology (2 mentions)
β actin, by Cell Signaling Technology (2 mentions)
Tnf α, by R&D Systems (2 mentions)
Ifn γ, by R&D Systems (2 mentions)
Ifn β, by R&D Systems (2 mentions)
2 protocols using ptyr690 stat2
1
Flow Cytometry and Western Blot Analysis of Interferon Signaling
2
Flow Cytometry and Western Blotting of IFN Signaling
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The following antibodies were used in flow cytometry: PE anti-mouse H-2Kb/H-2Db, PE anti-mouse IFNγR β chain (Biolegend, San Diego, CA, USA) and PE anti-mouse CD119 (IFNγ Receptor 1) (eBioscience, San Diego, CA, USA). Antibodies used in western blotting analysis were iNOS, pTyr701-Stat1, total Stat1, total Stat2, pSer536-p65, total p65 pSer32-IκBα, total IκBα, β-actin, GAPDH (Cell Signaling Technology, Danvers, MA, USA); pTyr690-Stat2 (Abcam, Cambridge, MA, USA) and LaminB (Epitomics, Burlingame, CA, USA). Recombinant mouse IFNα, IFNβ, IFNγ and TNFα were from R&D Systems (Minneapolis, MN, USA). l -NMMA was from Sigma-Aldrich (St Louis, MO, USA).
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