Bagmixer 400model vw

Aliquots of 10 mL were retrieved from each fermented flask of chokeberry juice after thorough homogenization at various time intervals during the 4 weeks at 4 °C. Each sample was serially diluted in 90mL of sterile Ringer’s solution of one-quarter strength (Sigma-Aldrich, Darmstadt, Germany), placed aseptically in a sterile plastic bag and homogenized in a Bagmixer (400 Model VW, Interscience). Subsequently the suspension was then subjected to serial decimal dilutions of one-quarter strength Ringer’s solution and plated on selective media.
Viable cell counts of the potentially probiotic strain (L. paracasei SP5) were enumerated on acidified MRS agar (Merck, Darmstadt, Germany) at 37 °C for 72h under anaerobic conditions (anaerobic jar, Anerocult C, Merck, Germany). Yeasts and fungi were enumerated on potato dextrose agar (PDA) after incubation at 30 °C for 72 h. Possible coliform cell counts were examined on Violet Red Bile Agar (VRBA) after incubation at 30 °C for 24 h [27 (link)].
All media were prepared according to the instructions provided by the manufacturer, were sterilized (120 °C, for 15 min, 1–1.5 atm), and then cooled to 45 °C prior to use. Cell counts were expressed as log of mean colony-forming units (cfu) per mL of chokeberry juice.