Adult worms were picked into S-basal buffer and lysed by addition of SDS sample buffer, followed by boiling for 15 min with occasional vortexing. Whole worm lysates were then separated on 4–12% polyacrylamide gradient gels (GenScript, #M00654), transferred to membranes, and blotted with mouse anti-HA (1:1000, Thermo Fisher, #26183), Guinea pig anti-HTP-3 (1:1500, MacQueen et al., 2005 (link)), mouse anti-α-tubulin (1:5000, Millipore Sigma, #05-829), rabbit anti-β-tubulin (1:2000, Abcam, ab6046), or HRP-conjugated anti-ALFA sdAb (1:1000, Nanotag Biotechnologies, N1505-HRP), HRP-conjugated anti-mouse secondary antibodies (Jackson Immunoresearch #115-035-068), HRP-conjugated anti-Rabbit secondary antibodies (Jackson Immunoresearch #111-035-144), HRP-conjugated anti-Guinea pig secondary antibodies (Jackson Immunoresearch #106-035-003). SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher, #34095) was used for detection.
Hrp conjugated anti guinea pig secondary antibodies
Manufactured by Jackson ImmunoResearch
HRP-conjugated anti-Guinea pig secondary antibodies are used to detect the presence of Guinea pig primary antibodies in various immunoassays. They contain horseradish peroxidase (HRP) enzyme conjugated to the secondary antibody, which can be used to generate a colorimetric or chemiluminescent signal for visualization and quantification of the target analyte.
Automatically generated - may contain errors
Lab products found in correlation
Rabbit anti β tubulin, by Abcam (2 mentions)
Hrp conjugated anti rabbit secondary antibody, by Jackson ImmunoResearch (2 mentions)
Hrp conjugated anti mouse secondary antibody, by Jackson ImmunoResearch (2 mentions)
Mouse anti ha, by Thermo Fisher Scientific (2 mentions)
Supersignal west femto maximum sensitivity substrate, by Thermo Fisher Scientific (2 mentions)
Guinea pig anti htp 3, by Merck Group (2 mentions)
Mouse anti α tubulin, by Merck Group (2 mentions)
2 protocols using hrp conjugated anti guinea pig secondary antibodies
1
Worm Protein Extraction and Western Blot
2
Worm Protein Lysate Preparation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Adult worms were picked into S-basal buffer and lysed by addition of SDS sample buffer, followed by boiling for 15 min with occasional vortexing. Whole worm lysates were then separated on 4-12% polyacrylamide gradient gels (GenScript, #M00654), transferred to membranes, and blotted with mouse anti-HA (1:1,000, Thermo Fisher, #26183), Guinea pig anti-HTP-3 [1:1,500, (MacQueen et al., 2005) (link)], mouse anti-α-tubulin (1:5,000, Millipore Sigma, #05-829), rabbit anti-β-tubulin (1:2,000, Abcam, ab6046), or HRPconjugated anti-ALFA sdAb (1:1,000, Nanotag Biotechnologies, N1505-HRP), HRPconjugated anti-mouse secondary antibodies (Jackson Immunoresearch #115-035-068), HRP-conjugated anti-Rabbit secondary antibodies (Jackson Immunoresearch #111-035-144), HRP-conjugated anti-Guinea pig secondary antibodies (Jackson Immunoresearch #106-035-003). SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher, #34095) were used for detection.
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