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L leucine d3

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L-Leucine-d3 is a stable isotope labeled amino acid. It is used as an internal standard in analytical methods for the quantification of leucine in various biological samples.

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Lab products found in correlation

Progesterone 2 3 4 13c3, by Merck Group (2 mentions) Milli q system, by Merck Group (2 mentions) Methanol meoh, by Thermo Fisher Scientific (2 mentions) Ms grade acetonitrile acn, by Thermo Fisher Scientific (1 mentions) L tryptophan d5, by Merck Group (1 mentions) Lc ms grade acetonitrile acn, by Thermo Fisher Scientific (1 mentions) L tryptophan d5, by LGC (1 mentions) Ammonium formate, by Merck Group (1 mentions) Formic acid, by Dikma Technologies (1 mentions)

2 protocols using l leucine d3

1

LC-MS Metabolite Quantification Standards

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MS-grade acetonitrile (ACN) and methanol (MeOH) were purchased from Thermo Fisher Scientific, USA. Formic acid was obtained from DIMKA, and ammonium formate was purchased from Fluka (Honeywell Fluka, USA). Ultrapure water was filtered through the Milli-Q system (Millipore, Billerica, MA). The internal standard (IS) mixture contained L-Leucine-d3 (Toronto Research Chemicals, TRC), L-Phenylalanine (13C9, 99%) (Cambridge Isotope Laboratories, CIL), L-Tryptophan-d5 (TRC), Progesterone-2,3,4-13C3 (Sigma).
Bai Y., Zhang H., Wu Z., Huang S., Luo Z., Wu K., Hu L, & Chen C. (2022). Use of ultra high performance liquid chromatography with high resolution mass spectrometry to analyze urinary metabolome alterations following acute kidney injury in post-cardiac surgery patients. Journal of Mass Spectrometry and Advances in the Clinical Lab, 24, 31-40.
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2

Metabolite Extraction for LC-MS Analysis

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LC–MS-grade acetonitrile (ACN) and methanol (MeOH) were purchased from Fisher Scientific, Inc. (Rockford, IL). Formic acid was obtained from DIKMA Technologies, Inc. (Beijing, China), and ammonium formate was purchased from Sigma Aldrich (St. Louis, MO). Internal standards L-Leucine-d3 and L-Tryptophan-d5 were from Toronto Research Chemicals (Toronto, ON); L-Phenylalanine (13C9, 99%) was from Cambridge Isotope Laboratories, Inc. (Tewksbury, MA); Progesterone-2,3,4-13C3 was purchased from Sigma Aldrich (St. Louis, MO). Ultrapure water was filtered through a Milli-Q system (Millipore, Billerica, MA).
Zhao Q., Hu Q., Meng S., Zhang Q., Wang T., Liu C., Liu D., Jiang Z, & Hong X. (2023). Metabolic profiling of patients with different idiopathic inflammatory myopathy subtypes reveals potential biomarkers in plasma. Clinical and Experimental Medicine, 23(7), 3417-3429.
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