MNP-based scaffolds were made from a slurry composed of aqueous suspensions of 1% w/v cellulose nanofibril (CNF) in water (University of Maine, Orono, Maine) that were thoroughly mixed with a known mass of freeze-dried dextran-coated iron oxide nanoparticles (micromod Partikeltechnologie GmbH, Rostock, Germany) under the name bionized nanoferrite (~100 nm diameter) to achieve concentrations of 25 mg Fe/mL or 250 mg Fe/mL (as measured by dividing the known mass of Fe contained in the measured scaffold volume). Bare scaffolds were made from a slurry of 1% w/v bovine tendon collagen (Worthington Biochemical Corporation, Lakewood, NJ) and 1% w/v nanocellulose blend of Bioplus™ nanocellulose fibrils and crystals (American Process, Atlanta, GA) mixed in a 50:50 v/v ratio.
Bovine tendon collagen
Manufactured by Worthington
Sourced in United States
Bovine tendon collagen is a naturally occurring protein extracted from the tendons of cattle. It serves as a versatile raw material for a variety of laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Elastin orcein, by Merck Group (1 mentions)
Ethylene glycol tetraacetic acid (egta), by Merck Group (1 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Tokyo Chemical Industry (1 mentions)
Gelatin, by Merck Group (1 mentions)
Cytochrome c, by Merck Group (1 mentions)
Casein, by Merck Group (1 mentions)
Aprotinin, by Merck Group (1 mentions)
2 protocols using bovine tendon collagen
1
Multimaterial Nanocomposite Scaffold Fabrication
2
Bovine Tendon and Bone Collagen Characterization
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Bovine tendon collagen was purchased from Worthington (Lakewood, NJ, USA), and bovine bone collagen from Kinry Biotech Co., Ltd. (Jinan, China). Casein (from bovine milk), gelatin (from cold water fish skin), elastin-orcein, EGTA, o-P, aprotinin, and cytochrome C were purchased from Sigma (St. Louis, MO, USA). Alcalase was purchased from Vazyme Biotech Co., Ltd. (Nanjing, China). PMSF was purchased from BBI (Shanghai, China). EDTA was purchased from HUSHI (Shanghai, China). Bacitracin was purchased from Aladdin (Shanghai, China). Tripeptide GGG and tetrapeptide GGYR were synthesized by Qiangyao Biotechnology Co., Ltd. (Shanghai, China). DPPH• was purchased from Tokyo Chemical Industry (Tokyo, Japan). HA was purchased from Shandong Freda Bioeng Co., Ltd. (Jinan, China). Other chemicals were of analytical grade and commercially available.
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