All the biological analytes, HSA, BSA, γ-globulin from human blood, fibrinogen from human plasma, transferrin from human, hemoglobin from human, haptoglobin from human, chymotrypsin from bovine pancreas, trypsin from bovine pancreas, lysozyme from pooled human plasma, and butyrylcholinesterase (BChE) from equine serum were purchased from Sigma-Aldrich (St. Louis, MO, USA) and used without further purification. High-throughput screening results were recorded by a Cytation 3 Multi-Mode Reader (BioTek, Winooski, VT, USA) using a 96-well plate. UV-vis spectra were recorded by a JASCO V-630 Spectrophotometer (JASCO Inc., Easton, MD, USA), and fluorescence spectra were recorded by a Cary Eclipse Fluorescence Spectrophotometer (Agilent, Santa Clara, CA, USA) using a quartz cell of path length 1 cm.
Butyrylcholinesterase bche from equine serum
Manufactured by Merck Group
Sourced in United States
Butyrylcholinesterase (BChE) from equine serum is a laboratory enzyme product. BChE is an important enzyme involved in the hydrolysis of the neurotransmitter acetylcholine. The product is extracted and purified from equine serum.
Automatically generated - may contain errors
Lab products found in correlation
Bovine serum albumin (bsa), by Merck Group (3 mentions)
Haptoglobin, by Merck Group (1 mentions)
Chymotrypsin, by Merck Group (1 mentions)
Fibrinogen, by Merck Group (1 mentions)
Cytation 3 multi mode reader, by Agilent Technologies (1 mentions)
γ globulin, by Merck Group (1 mentions)
Trypsin, by Merck Group (1 mentions)
Lysozyme, by Merck Group (1 mentions)
Transferrin, by Merck Group (1 mentions)
Human serum albumin (hsa), by Merck Group (1 mentions)
V 630 spectrophotometer, by Jasco (1 mentions)
Cary eclipse fluorescence spectrophotometer, by Agilent Technologies (1 mentions)
Acetylthiocholine iodide, by Merck Group (1 mentions)
Butyrylthiocholine iodide btci, by Merck Group (1 mentions)
Acetylcholinesterase ache, by Merck Group (1 mentions)
Trizma hydrochloride tris hcl, by Merck Group (1 mentions)
Milli q water purification system, by Merck Group (1 mentions)
5 5 dithiobis 2 nitrobenzoic acid dtnb, by Merck Group (1 mentions)
S butyrylthiocholine chloride, by Merck Group (1 mentions)
Formic acid, by Merck Group (1 mentions)
4 protocols using butyrylcholinesterase bche from equine serum
1
Analytical Characterization of Biomolecules
2
Acetylcholinesterase Activity Assay
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Acetylcholinesterase (AChE) from electric eel (Electrophorus electricus) (type VI-s, lyophilized powder), acetylthiocholine iodide (ATCI), butyrylcholinesterase (BChE) from equine serum (lyophilized powder), butyrylthiocholine iodide (BTCI), and 5,5′-dithio-bis-(2-nitrobenzoic acid) (DTNB) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Trizma hydrochloride (Tris-HCl) and bovine serum albumin (BSA) were obtained from Sigma-Aldrich (Steinheim, Germany). Deionized water was produced using a Milli-Q water purification system (Millipore, Bedford, MA, USA).
3
Amperometric Assay of Butyrylcholinesterase
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Commercial CB N220 of industrial standard grade was obtained from Cabot Corporation (Ravenna, Italy). Butyrylcholinesterase (BChE) from equine serum, bovine serum albumin (BSA), S-butyrylthiocholine chloride, 5,5’-dithio-bis-(2-nitrobenzoic acid) (DTNB), glutaraldehyde and paraoxon (paraoxon-ethyl), Nafion® (perfluorinated ion-exchange resin, 5% v/v solution in lower alcohols/water), acetonitrile, formic acid, ammonium formate (all HPLC grade) were purchased from Sigma Aldrich Company (St. Louis, MO, USA). Amperometric measurements were carried out using a portable PalmSens (Palm Instruments®, Houten, The Netherlands).
4
Colorimetric Assay for Butyrylcholinesterase
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Butyrylthiocholine chloride, butyrylcholinesterase (BChE, from equine serum), potassium ferricyanide (K 3 Fe(CN) 6 ), iron chloride (FeCl 3 ), urea, glucose, uric acid, serotonin hydrochloride, L-cysteine (L-Cys), L-glutathione reduced (GSH), glucose oxidase (GOx) and bovine serum albumin (BSA) were purchased from Sigma-Aldrich (St. Louis, MO). All reagents were used without further purification. Serum samples from volunteers were provided by University Hospital of Rome Tor Vergata. Optical measurements were carried by scanning the 96-well paper-based platform with a common office scanner (Canon PIXMA, MP450) with an optical resolution of 1200 × 2400 dpi, and the intensity of color was evaluated with the ImageJ software, an open source image-processing program. For BChE activity measurement by using reference method, MULTIGENT Cholinesterase method was employed by means of ARCHITECT System (Abbott) for clinical analyses located at University Hospital of Rome Tor Vergata.
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